摘要
目的建立裸鼠非小细胞肺癌(NSCLC)H460细胞动物模型,观察环氧化酶2(COX-2)选择性抑制剂塞来昔布(Celecoxib)对H460放疗的增敏作用,并对其作用机理进行初步探讨。方法建立Balb/c裸鼠肿瘤模型。动物随机分成3组:对照组、放疗组、塞来昔布+放疗组。灌肠法给予塞来昔布16mg/kg/d,4周时对比两组肿瘤瘤重;免疫组化方法分析共济失调-毛细血管扩张症突变蛋白(ATM)和表皮生长因子受体(EGFR)表达变化。结果 4周时塞来布昔+放疗组与放疗组的抑瘤效果比较有显著差异(P<0.05)。结论塞来昔布可能通过提高ATM和EGFR的表达,增强H460细胞的放疗敏感性,将来可能具有较好的临床应用价值。
Objective Nude mice model of nonsmall - cell lung cancer（ NSCLC ） H460 cell was established to inves-tigate the combined effects of radiotherapy and celecoxib. Methods Athymic mice bearing H460 were randomly divided into 3 groups： control, radiotherapy and radiotherapy plus celeeoxib group. 4 weeks after treatment,mice were killed to detect tumor weight. The expressions of ataxia-telangieetasia mutated（ATM） and epidermal growth factor receptor（EGFR） in tumor tis-sues were detected by immune - histoehmical （ S - P） method. Results The tumor weight of radiotherapy plus celecoxib group was significantly different with that of radiotherapy group （ P 〈 0.05 ） . The expressions of ATM and EGFR were significantly lower than that in radiotherapy group. Conclusion Celeeoxib promotes radiotherapeutic sensitivity of H460 by down - regula-ting the expression of ATM and EGFR. Celecoxib may presents potency in curing human lung cancer.
出处
《中国辐射卫生》
2013年第4期407-408,共2页
Chinese Journal of Radiological Health
基金
国家自然基金(31170804
31240052
31200634)
天津自然基金(11ZCGYSY02400
12JCYBJC15300
12JCYBJC32900)
关键词
塞来昔布
放射治疗
裸鼠
非小细胞肺癌
Celecoxib
Radiotherapy
Nude Mice
Nonsmall - Cell Lung Cancer
