摘要
目的评价PENTRA MS60血液分析仪(简称PMS60)对不典型淋巴细胞(ALY)检测的可靠性,为制订PMS60的复检标准提供依据。方法对PMS60检测到有ALY阳性报警的193例新鲜全血样本和无ALY报警的215例阴性样本分别制作血涂片,经瑞氏染色后进行显微镜镜检,408例新鲜全血样本同时用SYSMEXXS800i血液分析仪(简称XS800i)检测,对3种方法检测的ALY结果进行统计学分析。结果 PMS60与XS800i测定结果同时出现ALY报警阳性的一致率为89.7%,Kappa值为0.793;以显微镜分类为标准,PMS60提示ALY的Kappa值为0.792,显示一致性为满意,ALY报警敏感性为96.3%、特异性为85.3%、准确度为89.7%、约登指数为0.82。验证试验显示PMS60的准确度为94.6%。结论 PMS60提示ALY的可信性较高,当ALY比例>2.5%和/或ALY绝对值>0.25×109/L时必须进行显微镜分类。
Objective To evaluate the reliability of atypical lymphocyte （ALY） determination results by PENTRA MS60 hematology analyzer （PMS60） , and to provide the reference for the review criteria of PMS60. Methods The 193 cases of positive ALY and 215 cases of negative ALY from fresh whole blood samples were detected by PMS60 and prepared for blood smear, and microscopy classification was performed after Wright＇s staining. The 408 fresh whole blood samples were analyzed by SYSMEX XSS00i hematology analyzer （XS800i） simultaneously. The ALY determination results of the 3 methods were analyzed statistically. Results Compared the results of PMS60 and XS800i, the coincidence rate of positive ALY was 89. 7%, and the Kappa value was 0. 793. With microscopy classification as the standard, the Kappa value of PMS60 was 0. 792 with coincidence, the positive ALY sensitivity was 96.3%, the specificity was 85.3%, the accuracy was 89.7%, and the Yudon index was 0.82. After verification, the accuracy of PMS60 was 94.6%. Conclusions The ALY determination results of PMS60 are in high reliability, and it should perform microscopy classification when ALY proportion 〉 2.5% and / or ALY absolute value 〉 0.25 x 109/L.
出处
《检验医学》
CAS
2013年第8期659-661,共3页
Laboratory Medicine
