摘要
目的:研究软肝化坚颗粒药物血清对活化的肝星状细胞(HSC)信号传导通路的调节作用。方法:通过灌胃制备小鼠软肝化坚颗粒及秋水仙碱药物血清。将HSC-T6细胞分为5组,分别加入含5%、10%、20%软肝化坚颗粒血清,10%秋水仙碱血清和10%正常小鼠血清的培养基,培养24小时后,收集各组细胞。采用荧光定量RT-PCR检测Ⅰ型大麻素受体mRNA(CB1 mRNA)、黏着斑激酶mRNA(FAK mRNA)和细胞外信号调节激酶mRNA(ERKmRNA)水平。结果:与正常血清组相比,秋水仙碱组、5%、10%及20%软肝化坚颗粒血清组细胞中CB1 mRNA的含量均明显降低(P值均为0.000;与秋水仙碱组相比,10%、20%软肝化坚颗粒血清组细胞中CB1 mRNA的含量均明显降低(P值均为0.000)。与5%软肝化坚颗粒血清组相比,10%、20%软肝化坚颗粒血清组细胞中CB1 mRNA的含量均明显降低(P值均为0.000);与10%软肝化坚颗粒血清组相比,20%软肝化坚颗粒血清组细胞中CB1 mRNA的含量均明显降低(P值为0.000)。与正常血清组相比,秋水仙碱组、5%、10%及20%软肝化坚颗粒血清组细胞中ERK mRNA的含量均明显降低(P值分别为0.000、0.002、0.000和0.000);与5%软肝化坚颗粒血清组相比,20%软肝化坚颗粒血清组细胞中ERK mRNA的含量明显降低(P值为0.014)。与正常血清组相比,秋水仙碱组、5%、10%及20%软肝化坚颗粒血清组细胞中FAK mRNA的含量均明显降低(P值分别为0.001、0.000、0.000和0.000)。结论:软肝化坚颗粒可能通过ERK通路和PI3K-Akt通路抑制HSC-T6细胞活化,发挥防治肝纤维化的作用。
Objective： To study the influence of Ruangan Huajian granule on transforming signaling in the activation of he- patic stellate cells. Methods： Mouse medicated serum was prepared with Ruangan Huajian granule by gastrogavage. HSC-6 were divided into five groups： control group incubated with normal mouse serum （10%） ; Ruangan Huajian granule group incubated with Ruangan Huajian granule mouse medicated serum （5% , 10% , 20% ） ; colchicine group incubated with colchicine mouse medicated serum （ 10% ） . Ceils of each group were cultured for 24 hours, and cells were collected. The mRNA levels of CB1, FAK and ERK were determined by quantitative RT-PCR. Results： The levels of CB1 mRNA in colchicine group and Ruangan Huajian granule group （5% , 10%, 20% ） were notably lower than those in control group, P value was 0. 000, 0. 000, 0. 000 and 0. 000, respectively. The levels of CB1 mRNA in Ruangan Huajian granule group （ 10%, 20% ） were notably lower than those in colchicine group, P value was O. 000 and 0. 000, respectively. The levels of CB1 mRNA in Ruangan Huajian granule group （ 10% , 20% ） were notably lower than those in Ruangan Huajian granule group （5%）, P value was 0. 000 and 0. 000, respectively. The levels of CB1 mRNA in Ruangan Hnajian granule group （20%） were notably lower than those in Ruangan Huajian granule group （ 10% ）, P value was 0. 000. The levels of ERK mRNA in colchicine group and Ruangan Huajian granule group （5%, 10%, 20% ） were notably lower than those in control group, P value was 0. 000 , 0. 002, 0. 000 and 0. 000, re- spectively. The levels of ERK mRNA in Ruangan Huajian granule group （20%） were notably lower than those in Ruangan Hua- jian Particle group （5%）, P value was 0. 014. The levels of FAK mRNA in colchicine group and Ruangan Huajian granule group （5%, 10%, 20% ） were notably lower than those in control group, P value was 0. 001, 0. 000, 0. 000 and 0. 000, re- spectively. Conclusion： Ruangan Huajian granule can inhibit the act
出处
《中西医结合肝病杂志》
CAS
2013年第4期228-230,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
河北省中医药管理局科学技术研究计划基金项目(No.2008055)
关键词
软肝化坚颗粒
大麻素受体1
细胞外信号调节激酶
黏着斑激酶
肝纤维化
Ruangan Huajian granule
cannabinoid receptor 1
extracellular regulated kinase
focal adhesion kinase
hepatic fibrosis
