摘要
利用人工模拟酸性环境,从定期浇注HCl的土壤中筛选出一株脱氮硫杆菌T1菌株,并对其生长特性以及pH、盐度等理化因素对其脱氮除硫性能的影响进行了研究。结果表明,该菌株最适生长pH为7.02,最适脱氮pH为7.0;培养基中NaCl含量低于1.5%时,其脱氮能力没明显变化;与硫酸盐还原菌混菌培养能将H2S氧化为SO42-,从而明显抑制H2S的产生。在液体静置混菌培养过程中,在培养开始后的24h内,SO42-生成速率最大(11.21mg/(L·h)),随后不断降低。该菌不仅适用于不同pH、盐度废水的生物处理,还可以与硫酸盐还原菌混合添加到厌氧消化池中以减少硫化物的形成,从而减轻后者对污水处理系统中的金属设备和管道的腐蚀作用以及硫化氢对大气的污染。
In this study, a bacterial strain T1 was isolated from acid soil simulated in laboratory, and identified as Thiobacillus denitrficans. Its growth characteristics and the effects of some physical and chemical factors such as pH and salinity on its denitrification and desulphuration capacity were conducted. The results showed that the opti- mum growth pH ofT1 was 7.02 and the optimum denitrification pH was 7.0. When the NaC1 content in medium was lower than 1.5%, the denitrification ability didn't change obviously. As cultured with Sulfate-reducing bacteri- a, T1 can oxidize H2S into SO42 and distinctly reduce the production of hydrogen sulfide. In the static cultivation process of mixed .bacteria in liquid medium, the maximum rate of SO^- production is 11.21 mg/(L · h) in the first 24 hours and then the rate was decreased. It can be not only suitable for the biological treatment of wastewater with different pH or salinity, but also can reduce the production of sulfide by adding it to the anaerobic digester together with Sulfate-reducing bacteria, reducing the corrosion of metal equipment and pipeline in sewage treatment system and the air pollution by H2S.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2013年第4期449-452,共4页
Genomics and Applied Biology
基金
海口市重点科技计划项目(2009030)资助
关键词
脱氮硫杆菌
筛选
脱氮除硫性能
Thiobacillus denitrificans, Screening, Denitrification and desulfurization