摘要
目的:研究双参宁心方对大鼠心肌缺血/再灌注损伤能量代谢的影响。方法:采用Wistar大鼠结扎冠状动脉左前降支40 min,再灌注2 h,建立心肌缺血/再灌注模型,大鼠随机分为6组:假手术组、模型组、曲美他嗪组(10 mg·kg-1)、双参宁心方低、中、高剂量组(22.5,45,90 mg·kg-1)。预防给药5 d,每日1次,末次给药后1 h,行手术。肌酸激酶同工酶MB测定试剂盒检测血清CK-MB活性;HPLC测定心肌组织ATP,ADP,AMP含量并计算TAN和EC。结果:与模型组比较,双参宁心方预防性给药可降低血清CK-MB活性,同时增加心肌ATP含量和EC水平(P<0.01或P<0.05)。结论:双参宁心方通过保存缺血心肌ATP,维持心肌细胞能荷水平减轻缺血/再灌注损伤。
Objective： To investigate the effect of Shuangshen Ningxin（SSNX） formula on energy metabolism of myocardial is-chemia/reperfusion rats. Method： The myocardial ischemia/reperfusion model of Wistar rats was established through the ligation of left anterior descending branch of coronary artery of for 40 min and the reperfusion for 2 h. The Wistar rats were randomly divided into six groups： the sham operation group, the model group, the Trimetazidine group （ 10 mg·kg^-1 ） and SSNX groups （22. 5, 45, 90 mg·kg^-1 ）. Preventive administration was conducted for 5 d. The operation was performed at 1 h on the day of the last administration. CK-MB assay kit was adopted to detect the activity of serum CK-MB. HPLC was used to determine ATP, ADP and AMP contents in myo-cardial tissues and calculate TAN and EC. Result： The preventive administration with SSNX could reduce the activity of serum CK-MB and increase ATP content and EC level in myocardial tissues （P 〈 0. 01 or P 〈 0. 05 vs. the model group）. Conclusion： SSNX formula can maintain energy charge in cardiomyocytes and relieve ischemia/reperfusion injury by preserving ischemic myocardium ATP.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第17期2874-2877,共4页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2012ZX09103201-049
2012ZX09301002-004-002)
关键词
心肌缺血
再灌注
双参宁心方
能量代谢
HPLC
ischaemia/reperfusion
Shuangshen Ningxin formula
energy metabolism
HPLC
