摘要
目的 :观察煤烟颗粒诱发 DNA氧化损伤及其可能机制。方法 :应用 ESR波谱技术分析测定研究煤烟颗粒的自由基性质结构信息 ;应用 udenfriend反应体系研究其对 DNA氧化损伤作用。 结果 :ESR分析显示煤烟颗粒的 g值为 2 .0 0 16 ,近似半醌自由基 QH·性质 ;可诱发脱氧核糖 (d R)或脱氧腺苷 (d A)羟化降解 ,羟自由基清除剂 DMSO和甘露醇能抑制这种效应。燃油烟颗粒的 g值为 2 .10 0 8,宽谱 ,类似大气中飘尘自由基性质 ;不能诱发 d R和 d A的羟化降解作用。结论 :煤烟颗粒可诱发 DNA氧化损伤 ,其可能机制是煤烟颗粒中 QH·自氧化途径 (非酶反应 )。
Objective:To observe whether coal soot goes through non enzyme reaction pathway to produce ROS and cause DNA oxidative damage and its possible mechanism. Methods:Free radicals in coal soot were determined with electron spin resonance (ESR) spectrum technology. Free radicals to DNA oxidative damage were studied with udenfriend reaction system. Results: g =2.001 6 for coal soot from burning coal, and g=2.100 8 for smoke particles from burning oil. Coal soot could induce DNA hydroxylative degradation. Hydroxy radical scavengers such as DMSO and mannitol could inhibit the degradation.Conclusion:Coal soot can induce DNA oxidative damage. Its mechanism might be that QH · in coal soot goes through self oxidation pathway. [
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第9期874-876,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金!资助项目 ( 3 940 0 0 3 9)
