摘要
目的探讨HCV感染诱导自身免疫度应的机理。方法应用ELISA和流式细胞术检测了18例慢性丙型肝炎患者及16例正常人血清丙型肝炎病毒感染相关自身抗体（抗GOR）及抗核抗体（ANA）、外周血淋巴细胞（PBMC）亚群及其凋亡百分率。结果慢性丙型肝炎患者血清抗GOR抗体及ANA阳性率分别为66.7％和44.4％,与正常对照组比较差异均有显著意义（x^2＝26.86、x^2＝9.30，P＜0.005）。PBMC及CD_3~＋T淋巴细胞凋亡明显增加，与正常对照组比较差异有显著意义（t＝2．44，P＜0.05）。抗GOR阳性者CD_4~＋T淋巴细胞及CD_(19)~＋B淋巴细胞凋亡比抗GOR阴性者明显减少（t＝ 3．17P＜0.01；t＝2.294，P＜0.05）。抗GOR阳性者CD_4~＋CD_8~＋双阳性T细胞百分率较抗GOR阴性者显著增加（t＝2.44，P＜0.05），CD_3~＋／CD_4~＋T细胞显著降低（t＝2.18P＜0.05）。结论慢性丙型肝炎患者存在自身免疫，同时伴有外用血淋巴细胞亚群及其细胞凋亡的失衡。这种失衡可能是HCV感染诱导自身免疫的机理之一。
Objective To explore the autoimmune pathogenesis induced by hepatitis C virus. Methods Serumanti- GOR and peripheral blood lymphocyte subset and its apoptosis rate atier activated by Pokeweed mitogen (pwm ) in vitrowere measured by enzyme-linked immunosorbent assay and flow cytometry , respectively. Eighteen individuals with chronichepatitis C and 16 healthy controls were obserxed. Results The positive rates of serum anti-GOR (66.7% ) and ANA (44.4% )were significantly higher in chronic hepatitis C than normal controls (x~ 2 = 26.86. x~ 2 = 9.30. P < 0.005 ). The apoptosis per-centage of PBMCs and CD_3^+T cell was elevated in individuals with chronic hepatitis C when compared with normal con-trols (t = 2.44. P < 0.05 ). However. the apoptosis percentage of CD_4^+T and CD _19^+B cells in PBMCs was significantly de-creased in patients with anti-GOR positive compared to anti-GOR negative (t = 3. 1 7. P < 0.01; t = 2.294. P <0.05 ). Similarly.the percentage of CD_4^+CD_8^+T cells in anti-GOR positive patients was increased and CD_3^+/CD_4^+ was decreased (t = 2. 1 8. P<0.05) compared to anti-GOR negative (t = 2.44. P < 0.05; t = 2. 18. P <0.05 ). Conclusion Autoimmune response exists inchronic hepatitis C patients. There is an imbalance of lymphocyte subset and its apoptosis, which may be one of the impor-tant reasons for the mechanism of autoimmune pathogenesis of chronic hepatitis C.[
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第5期269-271,共3页
Chinese Journal of Hepatology
基金
霍英东青年基金!93065
