摘要
目的 探讨谷胱甘肽 S-转移酶 (GST- P)基因表达调控机制的多样性及其与化学致癌的关系 ,筛选与大鼠 GST- P增强子元件 GPE 相互作用的调控因子。方法 采用酵母单杂交体系筛选与 GPE 核心序列相互作用的转录激活因子 ,应用 DNA序列测定及计算机分析等手段对所测的 DNA序列进行分析。结果 共获得两个阳性克隆 p YGPE1和 p YGPE2。 DNA测序分析表明 :p YGPE1的插入片段与大鼠原癌基因 c- jun c DNA具有 99%的同源性 ,其编码的氨基酸序列与大鼠 c- Jun蛋白具有 10 0 %的同源性。 p YGPE2的插入片段与大鼠线粒体腺苷酸转位酶 c DNA具有 99%的同源性 ,其编码的氨基酸序列与大鼠腺苷酸转位酶具有 10 0 %的同源性。结论 大鼠 c- Jun蛋白和线粒体腺苷酸转位酶在酵母细胞内与大鼠 GST- P增强子 GPE 核心序列结合 ,可能是作用于 GPE 的反式作用因子。
Objective To investigate the diversity of the mechanisms of rat glutathione S transferase P(GST P) gene expression regulation and the relation between rat GST P gene expression and carcinogenesis. To search the trans action factors binding to the enhancer element of rat GST P (glutathione S transferase P enhancer, GPEⅠ). Methods We searched the trans action factors binding to GPEⅠ using yeast one hybrid system, and analyzed the DNA sequence by sequencing and computer search.Results Two positive clones pYGPE1 and pYGPE2 were obtained. The homologies of the insert sequence of pYGPE1 nucleotide and amino acid sequences with rat c jun cDNA were 99% and 100% respectively; the homologies of the insert sequence of pYGPE2 nucleotide and amino acid sequences with rat mitochondrial adenine nucleotide translocase cDNA were 99% and 100% respectively.Conclusions Rat c Jun and mitochondrial adenine nucleotide translocase can bind to the GPEⅠ core sequence, and they perhaps are the trans action factors of GPEⅠ.
出处
《中国医学科学院学报》
CSCD
北大核心
2000年第4期317-321,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金!( 3 9670 173 )资助&&
关键词
酵母单杂交体系
谷胱甘肽S-转移酶
增强子GPE
Ⅰ
yeast one hybrid system
glutathione S transferase P
glutathione S transferase P enhancer Ⅰ
trans action factors
