摘要
【摘要】目的了解E-钙粘蛋白在基因产物水平异质化表达对胃癌发生发展中的作用。方法以35例新鲜胃癌标本和10例癌旁组织标本中提取的DNA为模板，采用PCR-单链构象多态性分析法对E-钙粘蛋白的exon8和exon9的基因突变进行筛选，对筛选阳性标本行DNA序列测定进行确证：采用免疫组化法检测以上标本的E-钙粘蛋白表达情况；采用SPSS12．0统计软件进行数据分析。结果35例胃癌标本中有3例检出E-钙粘蛋白exon8突变，突变阳性率为8．6％（3／35），exon9未发现基因突变，检测出的突变全部位于分化不良型胃癌标本中。6例分化型胃癌和10例癌旁组织中未检出基因突变。E-钙粘蛋白在癌与癌周黏膜、肿瘤分化程度及Lauren不同分型中的表达差异均具有统计学意义（P均〈0．05）。结论E-钙粘蛋白是参与胃癌发生、发展的重要分子，其表达与胃癌的分化程度、浸润性生长、组织结构完整性和破坏程度等生物学行为具有较显著相关性，其发生基因突变是导致胃癌发生、发展的重要因素。
Objective To explore the effect of expression of E-cadherin in the level of gene products to gastric carcinoma＇s development. Methods 35 specimens from primary site of gastric carcinoma and 10 specimens from peripheral tissues of gastric tumor were detected. And the the E-cadherin gene mutation in ex- on8 and exon9 were screened by PCR-single strand conformationed polmorphism method, and the positive re- suits were confirmed by DNA sequencing. The protein expression of E-cadherin in these specimens were de- tected by immunohistochemistry. All data were analysed by SPSS 12.0 software. Results In 35 specimens, 3 specimens were detected E-cadherin gene mutation in exon8, the mutation rate was 8.6% （3/35）. There was no E-cadherin gene mutation in exon9. 3 specimens E-cadherin gene mutation in exon8 were undifferentiated specimens. E-cadherin gene mutation in 6 specimens differentiated gastric carcinoma and 10 specimens adja- cent gastric mucosa were not detected. The different expressions of E-cadherin in gastric carcinoma and mu- cous membrance of the gastric carcinoma, degree of tumor differentiation and Lauren types were all had statis- tical significance （Pall〈 0.05）. Conclusion E-cadherin is an important gene during the process of gastric carcinoma, significantly related with the degree of differentiation, infiltration growth ,the integrity of tissue structure of gastric cancer, and the mechanism of gene mutation. E-cadherin gene mutation is an important factor led to the progress of gastric carcinoma.
出处
《实用检验医师杂志》
2013年第1期14-18,共5页
Chinese Journal of Clinical Pathologist
