摘要
【摘要】目的探讨基于基因芯片的比较基因组杂交（array—basedcomparativegenomichybridiza—tion。Array—CGH）方法检测非特指型外周T细胞淋巴瘤（peripheralT—celllymphoma—nototherwisespeci—fled，PTCL—NOS）的分子遗传学改变特征的临床价值。方法收集2001年10月至2008年12月PTCL—NOS患者组织标本31例，采用1MbArray—CGH检测其基因变化，并用TilepathArray—CGH对检测结果进行验证。数据采用SPSS14．0统计软件进行分析。结果31例PTCL—NOS标本中，有17例（54．8％）出现不同程度的染色体改变，且有基因改变的患者生存期明显短于无基因改变的患者，差异均有统计学意义（P均〈0．05）。经TilepathArray—CGH检测验证，1MbArray—CGH检测的基因改变与TilepathAr—ray—CGH检测的基因改变完全一致。结论Array—CGH可以全面快速检测与肿瘤相关的染色体改变，对研究淋巴瘤特异性遗传特征具有重要意义。
Objective To analyze the genetic changes in peripheral T-cell lymphoma-not otherwise specified （PTCL-NOS） with array-based comparative genomic hybridization （Array-CGH）, and to discuss its clinical significance. Methods 31 tissue samples of PTCL-NOS patients were collected from October 200l to December 2008. The gene change in PTCL-NOS was detected by 1MB Array-CGH, and the results was veri- fied by Tile path Array-CGH. Data were analyzed by SPSS 14.0 statistical software. Results In 31 PTCL- NOS samples, there were 17 cases （54.8%） had gene changes in different levels. And the survival time of pa- tients with gene change were shorter than patients without gene change obviously, the differences all had sta- tistical significance （Pall〈 0.05）. The results of Tile path Array-CGH were exactly the same as that of 1Mb Array-CGH. Conclusion Array-CGH can analyze chromosomal changes related to the tumor and it＇s quite im- portant for the research of genetic imbalances of lymphoma.
出处
《实用检验医师杂志》
2013年第1期5-9,共5页
Chinese Journal of Clinical Pathologist
基金
国家自然科学基金资助项目（30771215）
