摘要
目的动态观察内源性大麻素受体l(CB1)在大鼠非酒精性脂肪性肝病(NAFLD)中的表达,初步探讨CB1在NAFLD发病中的作用。方法雄性SD大鼠48只,随机分3组:对照组(普通饲料)、模型组(高糖高脂饲料)、干预组(高糖高脂饲料+利莫那班),分别于实验第4、8周末每组各取8只处死,测体重、计算肝指数,检测血清ALT、AST、甘油三酯(TG)、总胆固醇(TC);ELISA法检测血清瘦素(Leptin)、肿瘤坏死因子(TNF)α含量,肝组织HE染色,观察其病理形态变化,免疫组化染色法观察NAFLD不同阶段肝组织CB1表达。采用单因素方差分析对各组间进行比较,组间两两比较采用SNK-q检验,组内比较采用两独立样本t检验,两指标间的相关性采用Pearson直线相关分析法。结果 (1)模型组大鼠血清ALT、AST、TC、TG、Leptin及TNFα水平均较对照组及利莫那班组高(P<0.05);(2)肝组织学改变:模型组第4周呈单纯性脂肪肝改变,第8周呈弥漫性肝脂肪变伴炎细胞浸润,干预组较同期模型组肝病变减轻;(3)肝脏CB1免疫组织化学检测:对照组表达阴性;模型组和干预组表达呈阳性。通过积分光密度值比较,模型组高于同期干预组(P<0.05)。结论 CB1在NAFLD患者中表达增强,并可能与TNFα和Leptin共同作用促进NAFLD进展。
Objective To observe the expression of endogenous cannabinoid receptor l（CB1） in rats with non-alcoholic fatty liver disease（NAFLD） and to preliminarily investigate the role of CB1 receptors in the pathogenesis of NAFLD.Methods Forty-eight male SD rats were randomly divided into three groups： control group（receiving normal feed）,model group（receiving high-sugar and high-fat feed）,and rimonabant group（receiving high-sugar and high-fat feed and rimonabant）.At the end of the 4th or 8th week of experiment,8 of each group were sacrificed.The body weight was measured,and the liver index was calculated.The serum levels of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,triglyceride（TG）,and total cholesterol（TC） were determined.The serum levels of leptin and tumor necrosis factor（TNF）α were determined by ELISA.The liver tissue was treated by HE staining,and the pathological changes were observed.Immunohistochemical staining was used to evaluate the CB1 receptor expression in liver tissues in different stages of NAFLD.One-way analysis of variance and t-test were used for within-group and between-group comparisons.Pearson′s linear-correlation analysis was used for evaluating the correlation between indices.Results The model group had significantly higher serum levels of ALT,AST,TC,TG,leptin,and TNF-α than the control group and rimonabant group（P0.05）.The model group showed simple fatty degeneration of the liver after 4 weeks and diffuse fatty degeneration of the liver with inflammatory cell infiltration after 8 weeks,while the rimonabant group had less severe liver lesions at the same time.CB1 receptor expression was not detected in the control group,but was detected in the model group and rimonabant group,according to the immunohistochemical staining,and the model group had a significantly higher integral optical density than the rimonabant group（P0.05）.Conclusion The expression of CB1 receptors is enhanced in patients with NAFLD,and it may act together w
出处
《临床肝胆病杂志》
CAS
2013年第8期611-615,共5页
Journal of Clinical Hepatology
关键词
脂肪肝
受体
大麻酚
CB1
瘦素
肿瘤坏死因子Α
fatty liver
receptor
cannabinoid
CB1
leptin
tumor necrosis factor-alpha
