摘要
目的：建立大体积固相萃取浓缩-超高效液相色谱（UPLC）串联质谱法快速测定11种常见全氟化合物的方法。方法：大体积水调pH近中性后，经WatersWAX固相萃取小柱富集净化，使用串联四极杆电喷雾离子源负离子扫描，多反应监测（MRM）模式检测，2mmol／L醋酸铵水溶液-2mmol／L醋酸铵甲醇溶液为流动相，内标法进行定量。结果：在1．0μg／L-50μg/L范围内的线性良好，11种全化氟化合物的方法最低检出限为0．02ng／L-0．5ng／L，回收率能达到71％～103％。结论：本方法操作简单、快速、自动实现对水中全氟化合物的测定。
Objective： To develop a method for rapid determination of 11 perfluorinated compounds （PFCs） in water by the Waters WAX solid phase extraction （SPE） and high performance liquid chromatography （UPLC） with MS/MS detector. Methods ： After adjusting the pH value to 7, the water was concentrated by the Waters WAX solid phase extraction（SPE）, and the perfluorinated compounds were detected in UPLC -MS/MS ESI（ - ） MRM mode and quantified by internal standard method. The mobile phase was a mixture of 2 mmol/L ammonium acetate aque- ous solution and 2 mmol/L ammonium acetate methanol solution. Results： There were good linearity between 1.0 μg/L - 50 μg/L, the detection limit of 11 kinds of perfluorinated compounds in water was 0.02 ng/L - 0.5 ng/L, the recoveries were between 71% -103%. Conclusion： It is simple, rapid and automatic for perfluorinated com- pounds determination in water.
出处
《中国卫生检验杂志》
北大核心
2013年第8期1831-1834,共4页
Chinese Journal of Health Laboratory Technology
基金
广东省科技计划项目(2012B031500018)
