摘要
目的检测非小细胞肺癌(NSCLC)患者癌组织与血浆中组织金属蛋白酶抑制因子-3(TIMP-3)、p16基因甲基化状态,研究血浆中两种基因的检测及联合检测在NSCLC筛查和鉴别诊断中的意义。方法采用甲基化特异性聚合酶链反应(MSP)技术,检测110例NSCLC患者癌组织、癌旁组织和外周血血浆TIMP-3、p16基因的甲基化状态,并对110例正常对照组血浆样品进行同样检测,比较各组检测结果。结果患者癌组织中TIMP-3、p16、联合检测的基因甲基化率分别高于癌旁组织(P<0.01);外周血血浆中TIMP-3、p16、联合检测基因甲基化率分别高于正常对照组血浆(P<0.01);血浆中TIMP-3、p16和联合检测的基因甲基化检出率与NSCLC临床分期、临床分类以及病理类型的对比差异均无统计学意义。结论对患者外周血浆TIMP-3、p16基因甲基化的联合检测可提高肺癌的检出率,为肺癌的筛查和鉴别诊断提供有价值的参考信息。
Objective To detect the methylation of the tissue inhibitor of metalloproteinases-3（TIMP-3） gene and p16 gene in the plasma and tissues of non-small cell lung cancer（NSCLC） patients and to evaluate its clinical significance in screening and differential diagnosis of NSCLC.Methods A methylation-specific polymerase chain reaction（MSP） was performed for detecting the methylation of the TIMP-3 and p16 in the lung cancer tissues,paraneoplastic tissues,and plasma from 110 NSCLC patients.The determination was also conducted on 110 normal blood samples.Results The total frequency of the TIMP-3 and p16 joint detection methylation in lung cancer tissues was significantly higher than those in the paraneoplastic tissues（P0.01）.The detection rate of hypermethylation for TIMP-3,p16 and joint detection in the plasma of the 110 NSCLC cases was also higher than those in the plasma of the control group（P0.01）.The single and joint detection rate of hypermethylation in the plasma did not significantly correlate with the clinical classification,the pathologic types,and clinical staging of the NSCLC.Conclusion Joint detection for hypermethylation of TIMP-3 and p16 genes can improve the positive rate,and provide valuable information for screening and differential diagnosis of the NSCLC.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第9期1088-1091,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:30471427)
安徽省重点科研项目(编号:07021017)
关键词
TIMP-3
P16
非小细胞肺癌
甲基化
甲基化特异性聚合酶链反应
tissue inhibitor of metalloproteinase-3
p16
non-small cell lung cancer
methylation
methylation-specific polymerase chain reaction
