摘要
目的:分析湖北省荆州地区类风湿关节炎(RA)患者人白细胞抗原DRB1(HLA-DRB1)等位基因阳性率及血管内皮细胞损伤标志物水平。方法:采用特异性引物聚合酶链反应(PCR-SSP)检测86例RA患者(RA组)和52例正常对照者(正常对照组)HLA-DRB1*0401-08、*0101-04、*1001等位基因;ELISA检测两组血清内皮细胞损伤标志物血管性血友病因子(vWF)及血清型血管内皮黏附分子(sVCAM-1)水平。结果:RA组和正常对照组均以*0405亚型的阳性率较高,且前者高于后者(P<0.05)。*0405和其它亚型基因型RA患者vWF及sVCAM-1水平显著高于正常对照组(P<0.05)。与*01或(和)*10亚型以及正常对照组比较,*04型RA患者sVCAM-1水平显著增高(P<0.05)。结论:本地区RA患者以HLA-DRB1*0405阳性率较高,且存在一定程度的血管内皮细胞明显损伤。
Objective: To analysis the human leukocyte antigen DRB1 (HLADRB1)alleles genotypes and the biomarkers of vascular endothelial injury of rheumatoid arthritis(RA) patientsin Jingzhou area of Hubei. Method: DNA amplification with sequence-specific primers PCR(PCRSSP)was used to determine HLA-DRB1*0401-08,*0101-04 and *1001 genotypes of 86 patients with rheumatoid arthritis and 52 normal controls. The concentrations of the biomarkers of vascular endothelial injury, vWF and sVCAM-1, were detected with enzyme-linked immunosorbent assay. Results: Compared with other genotypes, the frequency of DRB1*0405 was higher both in RA patients and normal controls. The frequency of DRB1*0405 in RA patients was higher than in normal controls (P<0.05). The concentrations of vWF and sVCAM-1 in RA patients of DRB1*0405 and other genotypes were higher than in normal controls (P<0.05).Compared with the * 01 or(and) *10 genotype group and normal controls,the concentration of sVCAM1 was higher in *04 RA patients(P<0.05). Conclusion: The frequency of HLADRB1*0405 genotype was higher in RA patients in this area. RA patients had vascular endothelial injury.
出处
《微循环学杂志》
2013年第3期52-54,I0002,共4页
Chinese Journal of Microcirculation
