摘要
目的:探讨依达拉奉对心梗大鼠梗死灶周边交感神经重构的作用及机制。方法:成年雄性SD大鼠70只,随机分成假手术组(n=10)、心梗组(n=30)、心梗+依达拉奉组(n=30)。心梗+依达拉奉组给予10mg/Kg依达拉奉腹腔注射,每天3次,注射4周;心梗组和假手术组给予等量生理盐水。给药时间从术后24h开始。术前及术后1周、2周、3周、4周末抽血检测血清丙二醛(MDA)、超氧化物歧化酶(SOD)水平。4周末比较各组大鼠存活率。之后处死大鼠,取出心脏,Masson染色检测心梗面积,免疫组织化学法检测交感神经生长相关蛋白43(GAP43)和络氨酸羟化酶(TH)抗体密度。结果:术后4周,与心梗组相比,心梗+依达拉奉组大鼠存活率显著提高(66.66%vs36.67%,P<0.05),血清MDA水平明显降低,SOD水平明显升高,梗死周边区交感神经GAP43和TH抗体密度显著降低(P均<0.05)。结论:依达拉奉能抑制心梗大鼠梗死灶周边交感神经重构,其机制可能与其抗氧化应激作用有关。
Objective: To investigate effect of edaravone on the sympathetic nerve remodeling in the infarct border zone after myocardial infarction (MI)in rats. Method: Seventy adult male Sprague Dawley rats were randomly divided into sham group, MI group and MI + edaravone group. MI + edaravone group were treated with edaravone (10mg/Kg ,intraperitoneal injection) , sham group and MI group were treated with vehicle (0.9% saline) three times a day for 4 weeks. Each treatment was started from 24 hours after operation. The levels of serum MDA and SOD was tested by ELISA before the operation and 1st week,2nd week,3rd week, 4th week after operation. The infarct size and desinties of GAP43/TH-immunoreactive positive nerves were tested by masson staining and immunohistochemistry respectively. Results: Four weeks after MI, the survival rate was significantly improved in the MI + edaravone group compared with that of MI group (66.66 % vs 36.67%, P< 0.05), the levels of serum MDA was decreased and the SOD was increased during the 1st week,2nd week,3rd week, 4th week. Moreover, the desinties of both GAP-43 and TH-immunoreactive nerves was attenuated by the edaravone after myocardial infarction (all P<0.05). Conclusion: Edaravone attenuate sympathetic nerve remodeling in the infarct border zone possibly by antioxidative stress.
出处
《微循环学杂志》
2013年第3期25-27,F0004,I0001,共5页
Chinese Journal of Microcirculation
关键词
依达拉奉
心肌梗死
交感神经重构
Edaravone
Myocardial infarction
Sympathetic nerve remodeling
