摘要
目的探讨多聚腺苷酸二磷酸核糖聚合酶（PARP）抑制剂3-氨基苯甲酰胺（3-AB）对重症急性胰腺炎（SAP）肾损伤的作用。方法雄性Wistar大鼠56只，分为7组：假手术组（简称SO组），SAP（3、6、12h）组，3-AB＋SAP（3、6、12h）组，每组8只。采用5％牛磺胆酸钠逆行胰胆管注射法制备SAP模型，3-AB＋SAP组在SAP造模前30rain经股静脉注射3-AB（20μg／g）。检测血清淀粉酶、肾功能、肾髓过氧化物酶（MPO）水平，光镜观察胰腺和肾脏病理变化，Westemblot检测肾组织PARP活化程度（PAR蛋白表达水平代表PARP活性程度）、细胞内黏附分子-1（ICAM-1）和P-选择素蛋白表达。多个样本均数比较采用单因素方差分析，两组间比较采用￡检验。肾损伤分级采用Kmskal-Wallis法秩和检验。结果SAP（3、6、12h）组血清淀粉酶分别为（3806±229）U／L、（4898±295）U／L和（5726±372）U／L，高于3-AB＋SAP相应时点组的（2785±160）U／L、（3241±198）U／L和（3953±249）U／L，差异有统计学意义（t=3．652，4．672，4．407，P〈0．05）；SAP（3、6、12h）组尿素氮分别为（11．6±0．8）mmo/L、（19．3±1．3）mmo/L和（29．6±2．1）mmo/L，均高于3-AB＋SAP相应时点组的（7．5±0．5）mmo/L、（10．5±0．7）mmo/L和（21．6±1．5）mmo/L，其中6、12h时相点两组比较，差异有统计学意义（t=3．836，6．849，P〈0．05）；SAP（3、6、12h）组肌酐分别为（48．7±3．1）μmol／L、（58．3±3．7）μmol／L和（75．9±5．4）μmol／L，均高于3-AB＋SAP相应时点组的（40．7±2．6）μmol／L、（43．2±2．6）μmol／L和（53．4±3．2）μmol／L，其中6、12h时相点两组比较，差异有统计学意义（t=3．279，3．073，P〈0．05）；SAP（3、6、12h）组肾脏MPO值分别为（0．69±0．06）U／g、（1．07±0．09）U／g和（1．42±0．13）U／g，均高于3-AB＋SAP相应时点组的（0．57±0．05）U�
Objective To investigate the eftects and mechanisms of poly adenosine diphosphate （ADP）-ribose polymerase inhibitor 3-aminobenzamide （3-AB） on kidney injury in rates with severe acute pancreatitis （SAP）. Methods Fifty-six male Wistar rats were divided into the sham operation （SO） group, SAP （3, 6, 12 hours） groups, and 3-AB ＋SAP （3, 6, 12 hours） groups, and there were 8 rats in each group. SAP model was established by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. Rats in the 3-AB ＋ SAP group were infused with 3-AB （20μg/g） via femoral vein 30 minutes before SAP model establishment. The serum amylase, kidney function and renal myeloperoxidase （MPO） were determined, and pathological scores of pancreatic and renal tissues were evaluated under light microscope. Renal poly ADP-ribose formation, intercellular adhesion molecules-1 （ICAM-I） and P-selectin expression were detected by the Western blot. All data were analyzed using the analysis of variance or t test. Renal injury grading was analyzed using the Kruskal-Wallis non- parametric test. Results The levels of serum amylase of SAP 3, 6, 12 groups were （3806 ± 229） U/L, （4898 ± 295）U/L and （5726 ±372）U/L, which were significantly higher than （2785 ± 160）U/L, （3241 ± 198）U/L and （3953 ± 249） U/L of the 3-AB ＋ SAP groups （ t = 3. 652, 4. 672, 4. 407, P 〈 0.05 ）. The levels of blood urea nitrogen were （ l 1.6 ± 0.8 ） mmol/L, （ 19.3 ± 1.3 ） mmol/L and （29.6 ± 2.1 ） mmol/L, which were higher than （7.5 ±0.5）mmol/L, （10.5 ±0.7）mmol/L and （21.6 ± 1.5）mmol/L of the 3-AB ＋SAP groups. There were significant differences in the levels of blood urea nitrogen between the SAP group and the 3-AB ＋ SAP group at the 6 and 12 hours （t =3. 836, 6. 849, P 〈0.05）. The levels of creatinine of the SAP 3, 6, 12 hours groups were （48.7 ±3.1） μmol/L, （58.3 ±3.7） μmol/L and （75.9±5.4） μmol/L, which were higher than （40.7 ±2.6�
出处
《中华消化外科杂志》
CAS
CSCD
北大核心
2013年第8期597-601,共5页
Chinese Journal of Digestive Surgery
基金
国家自然科学基金（81070368）
中央高校基本科研业务费专项资金（4104003）
