摘要
为了获得烟草NtNRT2.3基因的全长序列,进一步揭示其在烟草中的生物学功能,通过同源性搜索的方法,得到了烟草硝酸盐转运蛋白预测基因序列。采用RT-PCR技术,从烟草中成功克隆出一个硝酸盐转运蛋白体基因,将其命名为NtNRT2.3。其开放阅读框为1455bp,编码484个氨基酸。生物信息学分析表明,该基因与已报道的拟南芥、大豆、水稻等的NRT2家族基因具有高度同源性,且其编码的蛋白质具有NRT家族的共有结构特征。定量表达结果显示,NtNRT2.3基因在根中的表达量均高于茎和叶,表明NtNRT2.3基因在根系吸收硝酸盐过程中可能具有重要作用。
In order to obtain the full-length sequence of NtNRT2.3 and reveal its biological function in Nicotiana tabacum,a predicted nitrate transporter gene sequence in tobacco was gotten with homologous searching.By using RT-PCR technology,a nitrate transporter gene was successfully cloned from the Nicotiana tabacum and named as NtNRT2.3.Its open reading frame was 1455 bp and encoded 484 amino acids.Bioinformatics analysis showed that NtNRT2.3 was highly homologous to the reported NRT2 family genes in plants,such as Arabidopsis thaliana,soybean and rice.And its encoded proteins assumed the common structural characteristics of NRT family.The results of quantitative expression showed that the expression of NtNRT2.3 in roots was higher than that in stalks and leaves,which indicated that NtNRT2.3 gene might play an important role in nitrate absorption of root system.
出处
《烟草科技》
EI
CAS
北大核心
2013年第7期68-71,79,共5页
Tobacco Science & Technology
基金
中国烟草总公司重大科技专项"烟草功能基因筛选关键技术研究(110200902037)"
关键词
烟草
硝酸盐转运蛋白
克隆
表达
Tobacco
Nitrate transporter
Cloning
Expression
