摘要
目的 观察碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)对不同年龄白内障患者晶状体上皮细胞 (humanlensepithelialcells,HLEC)增殖的影响并探讨机制。方法 将白内障手术中取出的晶状体前囊膜在 10 μg/LbFGF中培养 48h ,免疫组织化学法染色后 ,采用医用多功能图像分析软件测定增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)阳性面积率 ;免疫组织化学方法测定晶状体前囊膜bFGF受体蛋白表达数量。结果 bFGF可促进不同年龄白内障患者HLEC增殖 ,其促增殖作用以及HLEC内bFGF受体蛋白的表达均与白内障患者年龄呈负相关 (P <0 .0 1)。结论 bFGF参与后发性白内障的形成 ;随着白内障患者年龄递减 ,bFGF促HLEC增殖作用增强 。
Objective To detect the age related effect of basic fibroblast growth factor (bFGF) on the proliferation of human lens epithelial cells (HLECs) in patients with cataract and discuss the mechanism Methods Human lens anterior capsules with attached HLECs were cultured in vitro, treated with 10 μg/L bFGF for 48hr, and then the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry was estimated Immunohistochemical analysis of bFGF receptor was also carried out. Results The bFGF could enhance the HLEC proliferation in patients with cataract at various ages The enhancement of the proliferation and the expression of bFGF receptor protein were negatively correlated to the age of the patients with cataract ( P <0 01) Conclusions The basic fibroblast growth factor might play an important role in the formation of posterior capsular opacity (PCO) after cataract surgery With the decrease in the patient's age, the enhancement of HLEC proliferation is increased, indicating that the fact is related to the high incidence of PCO in children
出处
《中华眼科杂志》
CSCD
北大核心
2000年第5期341-343,I020,共3页
Chinese Journal of Ophthalmology
基金
浙江省自然科学研究基金资助项目!(396497)
关键词
碱性成纤维细胞生长因子
上皮细胞
年龄
白内障
Fibroblast growth factor
basic
Epithelial cells
Lens
crystalline
Age related
