摘要
目的研究阿福豆苷（Afzelin）对家兔离体主动脉环的作用,并探讨其可能的作用机制。方法采用家兔离体主动脉环灌流模型,观察阿福豆苷对基础状态和去甲肾上腺素（norepinephrine,NE）预收缩主动脉环的作用,并观察不同抑制剂对阿福豆苷舒张血管作用的影响。结果阿福豆苷1×10-6~1×10-3mol.L-1对基础状态内皮完整的主动脉环的张力无影响;对NE预收缩的主动脉环,阿福豆苷在累积浓度3×10-6~1×10-3mol.L-1时能剂量依赖性和内皮依赖性地舒张主动脉环,并且该作用能分别被硝基-L-精氨酸甲酯（N-（omega）-nitro-L-arginine-methyl-ester,L-NAME）、亚甲蓝（methylene blue,MB）和吲哚美辛（indomethacine,Indo）预处理所抑制。结论阿福豆苷3×10-6~1×10-3mol.L-1对主动脉具有内皮依赖性的舒张作用,其舒张机制可能与一氧化氮-鸟苷酸环化酶-环磷酸鸟苷（NO-GC-cGMP）途径以及环氧合酶（COX）途径有关。
Objective To investigate the effect of Afzelin on isolated rabbit aortic rings and to explore the underlying mechanisms.Methods Isolated rabbit aortic rings perfusion model was established.The effect of Afzelin on the aortic rings in basal tension or in norepinephrine（NE）-induced contraction was observed.Three inhibitors were used to explore the mechanism.Results Cumulative administration of Afzelin（1 ×10-6~1 ×10-3 mol.L-1） had no effect on the vasomotion of aortic rings with endothelium in basal tension.The contracted arteries induced by NE could be dose-dependently and endothelium-dependently relaxed by Afzelin（3×10-6~1×10-3 mol.L-1）,while the effect was inhibited after preincubation with N-（omega）-nitro-L-arginine-methyl-ester（L-NAME）,methylene blue（MB） and indomethacin（Indo）,respectively.Conclusion The results indicated that the endothelium-dependent relaxation effect of Afzelin（3×10-6~1×10-3 mol.L-1）might be associated with nitric oxide-guanylate cyclase-cyclic guanosine monophosphate（NO-GC-cGMP）pathway and cyclooxygenase（COX）pathway.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2013年第4期341-344,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
科技部"十一五"国家科技支撑计划项目(2009BAI84B03)
