摘要
目的观察鞘内注射钙调蛋白依赖性激酶Ⅱ特异性抑制剂m—AIP对坐骨神经结扎大鼠疼痛感觉分辨和情绪体验的影响。方法sD雄性大鼠18只按随机数字表法分为3组（每组n=6）：假手术对照Sham组（S组）、坐骨神经结扎模型对照Control组（C组）、m-AIP组。C组和m—AIP组制备坐骨神经慢性挤压伤（CCI）模型，S组仅显露坐骨神经不结扎。术后7dS组和C组鞘内注射0．9％NaCl20μl，m-AlP组鞘内注射溶于0．9％NaCl的m—AIP0．5nmo/20μl。各组于给药后1．5h行反映痛情绪的大鼠位置逃避实验（Placeescape／avoidanceparadigm，PEAP），给药前及给药后2h、4h、8h检测大鼠的热缩足反射潜伏期（Pawwithdrawalthermallatency，PWTL）和机械性缩足反射阈值（Pawwithdrawalmechanicalthreshold，PWMT）。结果鞘内给予m—AIP0．5nmol能够改善大鼠痛行为反应，减少位置逃避行为。给药后2h、4hm—AlP组PwTL[（11．45±2．04）S，（10．26±1．48）S]，PWMT[（21．15±4．32）g，（20．45±4．09）g]比c组PwTL[（9．63±1．65）S，（9．30±0．73）S]，PWMT[（13．87±2．36）g，（14．804-3．12）g]升高，差异有统计学意义（P〈0．05）。给药前及给药后8hm—AIP组PWTL，PWMT与C组PWTL，PWMT相比，差异无统计学意义（P〉0．05）。结论CaMKII在坐骨神经痛的感觉分辨和情绪体验中起重要作用，鞘内注射m—AIP能够改善CCI大鼠痛行为和痛相关负性情绪。
Objective To investigate the sensory-discriminative and affective-motivational pain response of intrathecal injection of m-AIP, a special inhibitor of CaMKII, in a rat model of chronic constriction injury of sciatic nerve（CCI）. Methods Eighteen SD rats were divided randomly into 3 groups（n=6） ： Group S（sham） , Group C （control） and Group m-AIP. Group C and m-AIP were operated with the model of neuropathic pain induced by chronic constriction injury of sciatic nerve ; Group S were treated as sham operated rats. Seven days after operation, Group S and C received intrathecal injection of 0.9% NaCI 20 μ1, while Group m-AIP received intrathe- cal injection of m-AIP 0.5 nmo]/20 μl. Escape/avoidance behavior refrecting the affective-motivational dimension of pain was measured on 1.5 h after administration. Rats received pain behavior tests including paw withdrawal mechanical threshold（PWMT） and paw withdrawal thermal latency（PWTL） before and 2 h,4 h,8 h after adminis- tration. Results Treatment with m-AIP attenuated escape/avoidance behavior and reversed pain behaviors after CCI. At 2h and 4h after administration, Group m-AIP PWTL（ （ 11.45 ± 2.04） s, （ 10.26 ±1.48 ） s） and PWMT （ （21.15 ±4.32 ）g, （20.45 ±4.09 ） g） were increased when compared with Group C PWTL （ （9.63 ± 1.65 ） s, （9.30 ±.73） s） ,PWMT（ （ 13.87 ±2.36）g, （ 14.80 ±3.12）g） （P〈0.05）. Before and 8 h after administration, Group m-AIP PWTL, PWMT had no significant difference when compared with Group C （P 〉 0.05 ）. Conclu- sion CaMKII may play an important role in sensory and affective pain processing in neuropathic rats. Intrathecal injection of m-AIP can effectively improve pain behaviors and attenuate negative affect.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2013年第7期581-583,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金项目（81171047/H0903）
江苏省自然科学基金项目（BIL2010105）
江苏省卫生厅课题项目（RC2011006）
