摘要
目的 :研究体外培养的大鼠肠内菌对龙胆苦苷的代谢及大鼠灌服龙胆苦苷经体内肠菌代谢后血清中的药物成分变化。方法 :用HPLC法测定肠内菌培养液中和大鼠灌服龙胆苦苷后血清中的龙胆苦苷及其代谢产物龙胆碱、龙胆醛的含量。结果 :血清中第 2天检测到龙胆碱和龙胆醛 ,而龙胆苦苷的含量在第 3天呈下降趋势 ;肠内菌体外培养液中龙胆苦苷含量的下降和龙胆碱与龙胆醛含量的上升呈同步变化。结论 :龙胆碱和龙胆醛是龙胆苦苷体内代谢的重要生物活性产物。
Objective:To study the metabolism of gentiopicroside by rat intestinal flora in vitro and time profiles of gentiopicroside and its metabolites in rat serum after oral administration of gentiopicroside.Method:Using high performance liquid chromatography (HPLC) for determination of gentiopicroside, gentianine and gentianal content of rat intestinal flora culture medium in vitro and rat serum sample in vivo.Result:Gentiopicroside was metabolized quickly in vitro by rat intestinal flora, consequently gentianine and gentianal content appeared. Gentianine and gentianal were observed after gentiopicroside oral administration 4 times in rat serum sample, and gentiopicroside content decreased after oral administration 6 times.Conclusion:Gentianine and gentianal were the important bioactive metabolites of gentiopicroside in rats. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2000年第11期673-676,共4页
China Journal of Chinese Materia Medica
基金
国家"九五"攻关课题! ( 9690 3 0 2 0 3 )
关键词
龙胆苦苷
肠内菌群代谢
龙胆碱
龙胆醛
HPLC
gentiopicroside
intestinal flora metabolism
gentianine
gentianal
high performance liquid chromatography
