摘要
目的:探讨红景天苷对乙醇诱导的人L-02肝细胞氧化损伤的保护作用。方法:体外检测不同浓度梯度的红景天苷对人L-02肝细胞活性的影响;建立体外乙醇处理诱导的氧化损伤细胞模型,并以不同浓度红景天苷进行处理24h,检测肝细胞MDA水平、SOD和GSH-Px的活性。结果:与正常对照组相比,乙醇模型组MDA水平显著上升,SOD、CAT和GSH-Px的活性明显下降(P<0.05),经红景天苷处理,MDA水平下降,SOD和GSH-Px的活性活力显著上升(P<0.05)。结论:红景天苷可以减轻脂质过氧化程度,降低MDA含量,并提高肝细胞抗氧化的能力,使SOD和GSH-Px的活性升高,发挥了对肝细胞的保护作用。
Objective：To study protective effects of Salidroside on ethanol induced oxidative injury on human L-02 cell line. Methods. The cell model on ethanol-induced oxidative injury was established by culturing L-02 cell with 200mmol/ L ethanol in vivo for 24 hours as ethanol model groups. Half of ethanol group was treated with Salidroside at the same time as Selidroside-treated group. The SOD, GSH-Px and MDA in each group were detected. Results： The MDA in ethanol model group was significantly increased, but the activities of SOD and GSH-Px were decreased significantly than those in normal group respectively. After Salidroside intervention, the MDA was significantly decreased compared with the ethanol model group respectively. But the activities of SOD, and GSH-Px were increased significantly. Conclusion. Salidroside can protect enthanol induced oxidative injury in human L-02 cell line via alleviating lipid peroxidation and enhancing antioxidant abilities.
出处
《医学理论与实践》
2013年第15期1982-1983,1986,共3页
The Journal of Medical Theory and Practice
关键词
红景天苷
氧化应激
MDA
Salidroside, Oxidative stress, Malondiadehyde
