摘要
目的探讨肾炎康颗粒对慢性肾盂肾炎的治疗作用及其作用机制。方法制备大鼠单侧逆行性大肠杆菌感染慢性肾盂肾炎动物模型,肾炎康颗粒(0.125、0.25、0.5 g.kg-1)灌胃给药90 d后,进行尿和肾脏的细菌学、肾组织形态学、肾组织IL-1β和TGF-βmRNA表达的检测。结果尿液和肾组织无明显活菌存在,肾炎康颗粒可显著改善肾组织的病理损伤,减轻肾组织纤维化程度,降低肾组织IL-1β和TGF-βmRNA的表达。结论肾炎康颗粒对慢性肾盂肾炎大鼠具有较好的治疗作用,其机制可能与其通过降低肾组织TGF-β和IL-1βmRNA的表达抑制肾脏纤维化有关。
OBJECTIVE To study the effects of Shenyankang particle（SYK） in the chronic pyelonephritis（CPN）rats,and explore its potential mechanisms.METHODS Unilateral chronic nephropyelitis models were established by retroactive infection with colibacillus.SYK（0.125 g·kg-1,0.25 g·kg-1,0.5 g·kg-1）was administered by gavage.After dosed 90 d.Bacterial culture of urine and kidney,renal pathology were observed,and the mRNA level of IL-1β and TGF-β of kidney were detected by real time RT-PCR.RESULTS Viable organisms in urine and renal have not been seen,SYK significantly reduced the pathological injuries of kidney,lightened the degree of fibration of kidney,and decreased the mRNA level of TGF-β and IL-1β of kidney.CONCLUSION SYK had protective effects against CPN.The mechanisms appear to be due to the inhibited progression of fibrosis through suppression of TGF-β and IL-1β mRNA expression of kidney.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2013年第4期350-352,共3页
West China Journal of Pharmaceutical Sciences
基金
贵州省中药现代化科技产业研究开发专项项目(黔科合社字[2009]5020号)
贵州省科学技术基金项目(黔科合J字[2011]2317号)
