摘要
以感根茎腐烂病的樱桃树为试材,对其病原菌进行分离纯化、致病性检测和r-DNA-ITS序列分析,研究了pH和温度对病原菌菌丝生长的影响。结果表明:分离到的4株病原菌中仅1株引起了根茎腐烂病,经r-DNA-ITS序列分析鉴定,该菌为撕裂蜡孔菌;该菌在pH 4.0～7.0生长较快,最适宜pH为6.0;最适生长温度为32～34℃,最高生长温度38℃,致死温度42℃。
Taking cherry collar rot tree as material, the pathogen was isolated and purified, and its pathogenicity was tested and r-DNA-ITS sequence was analyzed. The effect of pH and temperature on growth of mycelium were also studied. The results showed that four strains were isolated but only one strain could cause cherry collar rot. The pathogen was Ceriporia lacerate based on r-DNA-ITS sequence analysis. The ideal pH for mycelium was 4. 0- 7. 0, the optimum pH was 6.0. The optimum growth temperature for the mycelium was 32-34℃ ,the maximum growth temperature was 38℃ and the lethal temperature was 42℃.
出处
《北方园艺》
CAS
北大核心
2013年第15期130-132,共3页
Northern Horticulture
基金
国家公益性行业(农业)科研专项资助项目(200903019)
烟台市科学技术攻关计划资助项目(2008107)
关键词
樱桃
根茎腐烂
撕裂蜡孔菌
cherry root crown rot
Ceriporia lacerate
