摘要
目的利用体外培养的皮肤原代成纤维细胞,造成紫外线(ultraviolet,UV)损伤模型,研究卵磷脂的抗氧化效果。方法紫外线照射正常人皮肤成纤维细胞诱发氧化损伤模型,UVA及UVB照射强度分别为5 J/cm2及0.6 J/cm2。测试组更换含1mg/ml油菜卵磷脂的培养液,同时设空白对照组、模型组和抗坏血酸阳性对照组。应用四甲基偶氮唑盐(microculture tetrazolium,MTT)比色法、二氯荧光素(2′,7′-dichlorodihydrofluorescin,DCFH)标记法和Annexinv/PI双染法,比较细胞活性、活性氧(reactive oxygen species,ROS)、超氧化物歧化酶(superoxide dismutase,SOD)和细胞凋亡等指标的变化。结果测试组SOD水平与照射组相比明显升高(P<0.05);测试组ROS荧光强度、细胞凋亡率显著降低(P<0.05)。结论紫外线可造成人皮肤成纤维细胞明显氧化损伤;油菜卵磷脂具有一定的抗氧化修复作用,抗氧化强度弱于维生素C。
Objective To induce ultraviolet （UV） damage model using primitive cultured fibroblasts in vitro and study the antioxidant effect of rapeseed lecithin. Methods The primary skin fibroblasts were cultured in vitro and then induced oxidation lesion model by UV-radiation with UVA and UVB dose at 5 J/cm2 and 0.6 J/era2 respectively. The cul- ture fluid in the test group was replaced by antioxidant medium contain lmg/ml rapeseed lecithin. The blank control group, radiation group, Vitamin C（VC） positive control group were set. The cell viability, reactive oxygen species （ROS）, superoxide dismutase （SOD） and cell apoptosis were measured by using microculture tetrazolium （MTT） assay,2＂, 7＂-di- chlorodihydrofluorescin （DCFH） labeling and Annexinv/PI fluorescein di-labeling method. Results The SOD level in the test group was significantly higher than that in the radiation group （P〈0. 05）. ROS fluorescence intensity and cell apopto- sis rate were significantly decrease in the test group （P〈0. 05）. Conclusion UV can induce the oxidative lesion of the pri- mary skin fibroblasts cells. Rapeseed lecithin has a certain antioxidant efficacy which is lower than that of VC.
出处
《华南国防医学杂志》
CAS
2013年第7期511-514,共4页
Military Medical Journal of South China
基金
国家质检总局科技计划项目(2012IK195)
关键词
油菜卵磷脂
抗氧化
紫外线损伤
Rapeseed lecithin
Antioxidant
Ultraviolet lesion
