摘要
目的研究人eag相关基因(human ether-a-go-go-related gene,HERG)蛋白对胃癌细胞增殖能力的影响。方法以基因重组方法构建HERG的siRNA质粒载体;然后将质粒载体转染胃癌细胞系,利用质粒抗性筛选稳定转染的细胞系,分别从蛋白和电流水平进行检测验证。采用四甲基偶氮唑盐(microculture tetrazolium,MTT)法对胃癌细胞进行生长曲线的描绘;采用平板克隆形成实验检测胃癌细胞的克隆形成能力。结果成功构建了HERG-siRNA载体,载体稳定转染胃癌细胞,转染细胞中HERG蛋白及相应电流均减弱,增殖能力减弱,克隆形成能力明显下降(P<0.05)。结论 HERG-siRNA可以抑制胃癌细胞的增殖和克隆形成能力,提示HERG电流在胃癌细胞的增殖中发挥作用,HERG蛋白是一个潜在的胃癌生物治疗的靶分子。
Objective To investigate the effect of human ether-a-go-go-related gene （HERG） protein on the prolif- eration of gastric cancer cells. Methods SiRNA vector of HERG was produced and transfected into gastric cancer cells. After transfection with HERG-siRNA, the proliferation curve of transfected gastric cancer cells was drawn with microcul- ture tetrazolium （MTT）, and the number of clone formation of transfected gastric cancer cells was calculated. Results The expression of HERG protein and HERG current in gastric cancer cells transfected with HERG-siRNA was decreased. The proliferation of gastric cancer cells and clone formation ability of gastric cancer cells were reduced （P〈0. 05） due to inhibiting HERG protein and current with siRNA. Conclusion HERG-siRNA can inhibit the proliferation and clone for- mation of gastric cancer cells. HERG protein is a potential target for gastric cancer biological therapy.
出处
《华南国防医学杂志》
CAS
2013年第7期451-454,505,共5页
Military Medical Journal of South China
基金
国家自然科学基金项目(30400204)
关键词
胃癌
人eag相关基因
钾离子通道
增殖
Gastric cancer
Human ether-a-go-go-related gene
Potassium channel
Proli{eration
