摘要
目的研究多发性硬化（ＭＳ）免疫异常过程中肿瘤坏死因子（ＴＮＦ－α）和循环细胞间粘附分子－１（ｃＩＣＡＭ－１）的变化及与血脑屏障（ＢＢＢ）的相关性。方法用酶联免疫吸附试验（ＥＬＩＳＡ）及单项琼脂扩散（ＳＩＲＤ）检测临床确诊为ＭＳ的３１例病人血清及脑脊液（ＣＳＦ）中ＴＮＦ－α、ｃＩＣＡＭ－１及白蛋白（Ａｌｂ）比值。结果①３１例ＭＳ患者中Ａｌｂ比值轻度异常８例（２５％），正常者２３例；ＩｇＧ指数增高（＞０．７）者２１例（６８％）。②ＭＳ组ＣＳＦ及血清中可检出高水平ＴＮＦ－α，较对照组显著增高（Ｐ＜０．０１，Ｐ＜０．０５）；ＣＳＦ中ｃＩＣＡＭ－１水平较对照组增高明显，差异有显著性（Ｐ＜０．０１）。③活动性ＭＳ患者ＣＳＦ中ＴＮＦ－α水平较静止期增高，差异有显著性（Ｐ＜０．０１）。两组ｃＩＣＡＭ－１水平也有显著差异（Ｐ＜０．０５）。④ＢＢＢ轻微损害 ＭＳ的 ＣＳＦ中 ｃＩＣＡＭ－１水平较 ＢＢＢ正常组增高，且与 ＣＳＦ中 ＴＮＦ－α水平呈正相关（ｒ＝０．４７８）。结论ＣＳＦ及血清中高水平ＴＮＦ－α与ｃＩＣＡＭ－１可能在ＭＳ病人鞘内炎症病理机制中起重要作用，ｃＩＣＡＭ－１可能成为ＭＳ活动的标志。一部分ＭＳ有一定程度ＢＢＢ损害且?
Objective To examine the changes of tumor necrosis factor-α(TNF-α) and circulating intercellular adhesion molecule-1 (cICAM-1 ) in patients with multiple sclerosis(MS) and the correlation with blood-brain barrier(BBB) .Methods Levels of cICAM-1 and TNF-α from 31 patients with clinically definite MS were detected by ELISA, and BBB function was evaluated by CSF/serum ratio and the paired CSF and serum samples. Results ①There were 8 mild abnormal(25. 8%) and 23 normal of Alb ratio in 31 MS patients. Twenty one patients (68% ) out of 31 were abnormal of IgG-Index(> 0. 7). ②In 31 MS patients, higher TNF-αlevels were determinated in serum and CSF, compared to control group(P < 0.05 and P < 0.01 respectively) .The level of cICAM-1 in CSF of MS patients was also elevated(P < 0.01). ③TNF-α level of CSF in active MS patients was higher than that in patients with stable MS.④The level of cICAM-1 in CSF of MS patients with mildly damaged BBB was higher than that in patients with normal BBB and associated with the level of TNF-α in CSF which showed a moderate correlation(r = 0.478). Conclusion The high levels of cICAM-1 and TNF-α may play an important role in intrathecal inflammatory pathogenesis in MS and cICAM-1 might be marker of activity in MS.
出处
《哈尔滨医科大学学报》
CAS
2000年第4期255-257,共3页
Journal of Harbin Medical University
关键词
多发性硬化症
肿瘤坏死因子
multiple sclerosis(MS)
blood-brain barrier(BBB)
tumor necrosis factor-α(TNF-a)
circulating intercellular adhesion molecule-1 (cICAM-1)
