摘要
目的建立女金片中丹皮酚高效液相色谱含量测定方法,为更好地控制女金片的质量提供参考。方法色谱柱:Agilent XDB C18柱(150 mm×4.6 mm,4.6μm);流动相:甲醇-水(45∶55);流速:1.0 mL/min;检测波长:274 nm;柱温:40℃。结果回归方程为Y=7×107X-16 965,r2=0.999 8。丹皮酚在0.003 8～0.38μg范围内线性关系良好,平均回收率为99.7%,RSD=1.56%(n=6)。结论该方法简便、准确、重复性好,能够为女金片的质量控制提供参考。
Objective To provide a better reference for the quality control of Nujin Pian by establishing the method for the determination of paeonol in Nujin Pian by HPLC.Methods HPLC was used Agilent XDB C18 column（4.6 mm×150 mm,4.6 μm）,mobile phase was composed of methanol-water（45：55）.The flow rate was 1.0 mL/min with detection wavelength at 274 nm and column temperature at 40 ℃.Results The regression equation of paeonol was Y=7×10^7X-16 965,r 2=0.999 8（n =6）.The linear range of paeonol was 0.003 8-0.38 μg.The average recovery rate was 99.7%,and RSD was 1.56%（n =6）.Conclusion The method is simple,fast and accurate with good reproducibility.It can provide reference for the quality control of Nujin Pian.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第8期59-60,共2页
Chinese Journal of Information on Traditional Chinese Medicine
