摘要
目的 研究脑活素 (现药名施普善 )对原代培养的大鼠小脑颗粒神经元的影响。方法 采用原代培养的大鼠小脑颗粒神经元 ,观察脑活素对细胞形态学及生存的影响。用噻唑蓝 (MTT)法测定细胞存活率。结果 脑活素在 0～ 40mL/L浓度下 ,剂量依赖性诱导小脑颗粒神经元死亡。兴奋性氨基酸受体拮抗剂地佐环平 (MK -80 1)可保护低浓度脑活素 (<5 0mL/L)的神经毒性。脑活素浓度 >5 0mL/L时MK - 80 1无明显保护作用。而对照药物胞二磷胆碱 (0～ 10g/L)、单唾液酸四己糖神经糖甙脂 (GM - 1,0～ 2 0mg/L)两者被认为具有护脑作用的药物 ,对小脑颗粒细胞均无毒性作用。结论 脑活素对原代培养的大鼠小脑颗粒神经元具有毒性作用 ,其机制部分是通过兴奋性氨基酸介导的。
Objective To investigate the effect of cerebroysin on primary cultured rat cerebellar granular nearons.Methods Primary cutured rat cerebelar granule neurons were exposed to cerebrolysin and the survival of cells and cell morphology were observed.The neuronal survival was examined by MTT assay.Results At the concentration of 0～40mL/L,cerebrolysin may induce the death of cerebellar granule neurons in a dose-dependent manner.MK-801,a non-competitive excitatory amino acid receptor antaginist,can protect the neurons from the toxicity induced by low concentrated cerebrolysin(<50mL/L),but failed to work in high cerebrolysin concentration(>50mL/L).cerebelar granule neurons were also exposed to citicoline (0～5g/L) and GM-1 (0～20mg/L),both of which are supposed to be the protectors of the brain and were served as control in this study.No effects on neuronal survival were found in ether of the drugs.Conclusion The neurotoxicity induced by cerebrolysin was partly mediated by excitatory amino acids in its solutions.It is of value to investigating the pharmacological effects of cerebrolysin on central nervous system further.
出处
《中国实用儿科杂志》
CSCD
北大核心
2000年第8期464-466,共3页
Chinese Journal of Practical Pediatrics
基金
国家自然科学基金 !(NO :39770 782 )
广东省自然科学基金 !(NO :970 0 5 2 )资助
