摘要
在Lipozyme 435脂肪酶催化下,利用异VC与山茶油、猪油、麻油和菜籽油分别发生酯交换反应,合成异VC混合脂肪酸酯,产物用柱层析法分离纯化。以山茶油与异VC反应为例,详细考察了反应溶剂、反应时间、反应物配比、酶用量和油脂回收循环使用对反应转化率的影响。优化条件下,反应转化率最高达到48%。将合成的异VC山茶油脂肪酸酯、异VC猪油脂肪酸酯、异VC芝麻油脂肪酸酯和异VC菜籽油脂肪酸酯应用于新鲜山茶油中,用过氧化值法(POV)进行抗氧化试验。结果表明,异VC混合脂肪酸酯是良好的脂溶性抗氧化剂,并表现出明显的剂效关系。
D-isoascorbyl mixed-fatty acid esters were synthesized with Lipozyme 435 as catalyst by the transesterification of D-isoascorbic acid with camellia oil,lard,sesame oil and canola oil,respectively.The effect of factors such as reaction solvent,reaction time,the ratio of reactants,the amount of lipase,and the recycled oils and fats on the reaction conversion were explored with the synthesis of D-isoascorbyl camellia oil fatty acid esters as reaction model to optimize the reaction conditions.Under the optimized conditions,the reaction conversion could reach 48 %.The antioxidation activities of D-isoascorbyl camellia oil fatty acid esters,D-isoascorbyl lard fatty acid esters,D-isoascorbyl sesame oil fatty acid ester and D-isoascorbyl rapeseed oil fatty acid ester in camellia oil was tested by POV determination,respectively.The results showed that all the synthesized D-isoascorbyl mixed-fatty acid esters were excellent fat-soluble antioxidants and that their antioxidation activities increased with the applied concentration.
出处
《食品研究与开发》
CAS
北大核心
2013年第14期6-9,共4页
Food Research and Development
基金
江西省普通高校重点实验室科技计划项目(GJJ08523号)
江西省科技条件平台建设项目(2010DTZ01900)
关键词
异VC
脂肪酶
异VC混合脂肪酸酯
油脂
抗氧化性
D-isoascorbic acid
lipase
D-isoascorbyl mixed-fatty acid ester
oils and fats
antioxidation
