摘要
目的:研究早期干预对缺氧缺血性脑损伤(Hypoxic-ischemic brain injury)新生大鼠内源性神经干细胞分化能力的影响。方法:共75只7日龄SD(Sprague Dawley)大鼠,随机分成模型组(n=50)和假手术组(n=25),模型组用Rice法制作50只缺氧缺血性脑病模型,分成HIBD组(n=25)和干预组(n=25)。对干预组进行早期干预,HIBD组和假手术组常规饲养,每组选取1天,7天,14天,21天,28天5个时间点处死5只,每只鼠处死前都进行腹腔BrdU注射标记新生细胞,应用荧光免疫双标技术检测各时间点海马齿状(DG)GFAP/BrdU和NeuN/BrdU阳性细胞数。结果:(1)3组大鼠DG区均有BrdU/NeuN和BrdU/GFAP阳性双标记染色;(2)与假手术组比较,干预组及HIBD组大鼠DG区BrdU/NeuN和BrdU/GFAP阳性双标记染色在术后14,21,28天均有显著增高(P<0.01)。(3)早期干预组大鼠术后14,21,28天时的BrdU/NeuN和BrdU/GFAP阳性双标记染色均显著高于HIBD组(P<0.01)。结论:缺氧缺血致大鼠脑损伤可诱导内源性神经干细胞分化;早期干预可提高脑损伤鼠内源性神经干细胞分化能力。
Objective： To study the impact of early intervention on differentiation of neural stem cells （NSCs） in brain damage rats af- ter HIBD （Hypoxic -ischemic brain injury）. Methods：Totally 75 SD （Sprague Dawley）rats aged 7 days were randomly divided into two groups： HIBD model （ n = 50） and sham - operated （ n = 25 ）. 50 HIBD model was produced with Rice method, and randomly divided into HIBD group（ n = 25 ） and early intervention group（ n = 25 ）. HIBD group and sham -operated group are normally feed, intervention group are dealed with early intervention,5 rats were killed at 1 day ,Tdays, 14days,21 days and 28days after operation in each group. Each rat was injected with BrdU into its abdominal cavity to mark new cells before killed. Double staining immunofluorescence was used to detect the co - expression of bromodeoxyuridine （BrdU） with neuronal nuclei antigen （NeuN） or glia fibrillary acidic protein （GFAP） in the Dentate gyrus（DG） at different time points. Results： （1）BrdU/NeuN and BrdU/GFAP double staining cells were observed in all 3 groups. （2） In the HIBD and intervention groups, at 14, 21 and 28 days after the surgery, the number of BrdU/NeuN and BrdU/GFAP double - stained cells increased in the DG compared with the sham - operated group（ P 〈 0.01 ）. （3） Obviously more BrdU/NeuN and BrdU/GFAP double - stained cells were found in the early intervention group than that in the HIBD group at 14, 21 and 28days（ P 〈 0.01 ）. Conclu- sion：Our results indicate that the brain damae caused＇by HIBD can induce the differentiation of endogenous NSCs; Early intervention can increase the differentiation of endogenous NSCs.
出处
《中国伤残医学》
2013年第7期97-99,共3页
Chinese Journal of Trauma and Disability Medicine
关键词
缺氧缺血性脑病
神经干细胞
干预
大鼠
动物
实验
HIBD（ Hypoxic- ischemic brain injury）
NSCs （neural stem cells）
Intervention
Rat
Animal experiment
