摘要
选择青藏高原广泛推广的10个青稞品种的成熟胚作为外植体,通过诱导愈伤组织和不定芽,以及生根培养等,建立了青稞植株的再生体系。结果表明,在添加L-脯氨酸1g·L-1+水解酪蛋白0.8g·L-1+2,4-D2mg·L-1的MS培养基上愈伤组织诱导率较高;诱导不定芽效果较好的培养基为MS+L-脯氨酸1g·L-1+水解酪蛋白0.8g·L-1,不定芽诱导率超过90%;将不定芽转到1/2MS培养基上进行生根培养,1个月后可获得完整植株。
For highland barley plant regeneration,mature embryos selected from 10 popularized varieties of high-land barley （ Hordeum vulgare L. var. nudum Hook. f. ） in Qinghai-Tibet Plateau were used as explants. A plant regeneration system was successfully established via regular tissue culture procedure. In the plant regeneration system the callus inducing rate for the embryos of the 10 barley varieties was higher when cultured in the MS medium at the presence of L-Proline （ 1 g·L^–1 ） ＋ Casein hydrolyzate （ 8 g·L^–1 ） ＋ 2,4-D （ mg·L^–1 ） , and adventitious bud inducing rate was higher in the MS medium supplemented with L-Proline （ 1 g·L^–1 ） ＋ Casein hydrolyzate （ 8 g·L^–1 ） . The plantlets were induced to root in the 1/2 MS rooting medium.
出处
《热带生物学报》
2013年第2期129-132,共4页
Journal of Tropical Biology
基金
中国农业大学农业生物技术国家重点实验室开放课题(2008SKLAB08-04)
关键词
青稞
Β-葡聚糖
植株再生
Hordeum vulgare var. nudum
β-glucan
plant regeneration
