摘要
目的以人源菌群(HFA)小鼠为研究模型,观察抗性淀粉(RS)对高脂饮食诱导的肥胖小鼠肠道菌群的多样性的影响。方法将30只无菌小鼠接种健康人志愿者的粪便悬液构建HFA小鼠模型后,随机分成3组,一组喂养含20%的抗性淀粉的高脂饲料(RS组),一组喂养纯高脂饲料(CK组),一组喂养普通饲料(CONV组),取第0周和第8周的小鼠新鲜粪便,用PCR-DGGE分析3组小鼠的肠道菌群的相似性和多样性。结果 3组小鼠在第0周时肠道菌群多样性的相似度达到79%～87%,与人的肠道菌群相似性达到39%,说明构建HFA小鼠模型成功,第8周时,3组之间的均匀度(E)和Shannon指数差异无统计学意义(P>0.05),而丰富度(S)在高脂组(CK)与普通饲料组(CONV)和抗性淀粉组(RS)之间差异都有统计学意义(P<0.05),说明高脂饮食引起肠道菌群多样性增加,而抗性淀粉则能降低这种多样性。结论抗性淀粉可以显著影响HFA小鼠的肠道菌群多样性。
Objective To study the effects of resistant starch（RS） on the diversity of intestinal microflora molecular profile in the fecal samples.Methods 30 germfree mice were inoculated with fecal suspension derived from a healthy volunteer to construct an HFA mouse model.The HFA mice were randomly divided into three groups： one group was fed with a high fat diet containing 20% resistant starch（RS group）,another group was just fed with pure high fat diet（CK group）,and the last group was fed with normal diet（CONV group）.The similarity and diversity of the intestinal flora from Week 0 and Week 8 in the fresh fecal samples of the three groups were analyzed by PCR-DGGE method.Results The similarity of the intestinal flora diversity among the three groups of mice at Week 0 was from 79% to 87%,and the similarity with the human volunteer＇s intestinal flora was 39%.This means that an HFA mouse model was built successfully.At Week 8,the degree of evenness（E） and Shannon index among the three groups were not significantly different（P0.05）.There was a significant difference（P0.05） on the richness（S） among the CK,CONV and RS group.The high-fat diet had increased the intestinal flora diversity,while resistant starch lowered it.Conclusion Resistant starch has an effect on the intestinal flora diversity in HFA mice.
出处
《中国微生态学杂志》
CAS
CSCD
2013年第7期762-765,共4页
Chinese Journal of Microecology
基金
国家自然科学基金(31171673
31071528
31271956)
国家重点基础研究发展计划(2013CB531406)
