摘要
目的:观察锌离子螯合剂TPEN对脂多糖(LPS)刺激下小鼠BV2小胶质细胞核转录因子NF-кB P65亚基磷酸化及诱导型一氧化氮合酶(iNOS)mRNA表达的影响。方法:体外培养BV2细胞,用不同药物进行孵育,分组如下:正常对照组,单独LPS组,TPEN与LPS共处理组,NF-кB抑制剂BAY11-7082和LPS共处理组。采用实时荧光定量PCR法检测iNOS mRNA表达,Western Blot法检测磷酸化NF-кB P65(p-P65)蛋白表达。结果:TPEN浓度依赖性地减少LPS诱导的p-P65蛋白和iNOS mRNA表达,BAY11-7082浓度依赖性抑制LPS诱导的iNOSmRNA表达的上调。结论:TPEN能够减少LPS诱导的BV2细胞iNOS mRNA表达,该作用可能与其抑制NF-кB信号通路的激活有关。
Objective： To study the effects of zinc chelator TPEN on lipopolysaccharide-stimulated phosphorylation of nuclear transcription factor-kappaB （NF-KB） P65 subunit and inducible nitricoxide synthase （iNOS） mRNA expression in mouse BV2 microglial cells. Methods： BV2 microglial ceils were cultured in vitro, incubated with different drugs and divided into the following groups ： normal control group, single LPS-treated group, TPEN and LPS eotreated group, inhib- itor for NF-xB BAY11-7082 and LPS cotreated group. The mRNA expression of iNOS was analyzed by real-time PCR. The protein expression of phosphor-NF-KB P65 （p-P65） was examined by Western Blot. Results： TPEN concentration- dependently reduced LPS-indueed p-P65 protein and iNOS mRNA expression. BAY11-7082 inhibited LPS-induced upreg- ulation of iNOS mRNA in a concentration-dependent manner. Conclusion： TPEN inhibits LPS-induced iNOS mRNA ex- pression, which may be associated with its suppressing the activation of NF-KB signal pathway.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2013年第4期410-414,共5页
Chinese Journal of Neuroanatomy
基金
南通市科技计划项目(BK2011049)
南通市科技计划项目(BK2011009)
