摘要
目的通过研究苯并[a]芘（B[alP）对大鼠行为学、海马氧化应激及ATP酶的影响，探讨B[alP的神经行为毒性分子机制。方法将120只21d龄雄性SD大鼠，随机分为空白对照组、植物油组（溶剂对照组），2．5、5．0、10．0mg／kgB[a]P染毒组，每组24只。腹腔注射给药，每天1次，连续4周。染毒结束后，用Morris水迷宫和穿梭箱检测学习记忆能力；用化学比色法测定海马超氧化物歧化酶（SOD）、Na‘K’ATP酶和Ca。‘Mg。‘ATP酶的活力及丙二醛（MDA）含量；用荧光标记方法测定海马ca：＋浓度。结果各染毒组大鼠的水迷宫逃避潜伏期、穿梭箱主动回避反应潜伏期（AARL）和被动回避反应潜伏期（RARL）均明显高于空白对照组和溶剂对照组。水迷宫末次跨平台次数和穿梭箱主动回避反应次数（AARF）均明显低于空白对照组和溶剂对照组，差异均有统计学意义（P〈0．05）；且呈剂量一效应关系。与空白对照组和溶剂对照组比较，染毒组大鼠海马组织SOD活力、Na‘KTP酶和Ca2,Mg“ATP酶活力明显下降．且呈剂量一效应关系，差异均有统计学意义（p〈O．05）。染毒组大鼠海马组织MDA含量、Ca2＋度均明显高于空白对照组和溶剂对照组，且呈剂量一效应关系，差异均有统计学意义（P〈O．05）。结论BMP所致神经行为毒性，可能与染毒后大鼠海马组织氧化应激受损，Na”ATP酶和cpMgnATP酶活力下降有关，
Objective To investigate the effects ofbenzo[a]pyrene （B[a]P） exposure on the behaviors and hippocampal oxidative stress and ATPase in rats and the molecular mechanism of neurobehavioral toxicity of B[a]P. Methods A total of 120 male SD rats （21 days old） were randomly and equally assigned to five groups： blank control group, vegetable oil （solvent control） group, and 2.5, 5, and 10 mg/kg B[a]P exposure groups. The rats in B [alP exposure groups were injected intraperitoneally with B[a]P once a day for 4 consecu- tive weeks. Then, Morris water maze and shuttle box were used to evaluate the learning and memory abilities of rats; colorimetric assay was used to measure the activities of superoxide dismutase （SOD）, Na＋/K2-ATPase, and Ca27Mg2＋-ATPase and the content of rualonaldehyde （MDA） in the hippocampus; the concentration of Ca2＋ in the hippocampus was measured by fluorescent labeling. Results Compared with the blank control group and solvent control group, the B[a]P exposure groups exhibited significant increases in escape latency, active avoid- ance response latency, and passive avoidance response latency and significant decreases in number of platform crossings and active avoidance response frequency in the last test （P〈0.05 for all comparisons）, with a dose-ef- fect relationship. In addition, the B[a]P exposure groups had significantly lower activities of SOD, Na＋/K＋-AT- Pase, and Ca27Mg2＋-ATPase and significantly higher MDA level and Ca2. concentration than the blank control group and solvent control group （P〈0.05 for all comparisons）, with a dose-effect relationship. Conclusion The neurobehavioral toxicity of B[a]P may be related to increased oxidative stress and decreased activities of Na＋/K＋- ATPase and CaZTMgZ-ATPase in the hippocampus of rats.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2013年第7期500-503,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
围家自然科学基金（30671744）
