摘要
本试验旨在研究三维模式下培养时间对奶牛乳腺上皮细胞(BMECs)酪蛋白基因表达的影响。采用3头健康的3～5岁泌乳黑白花奶牛的乳腺组织,将BMECs经1代纯化后进行三维培养,在三维模式下分别培养3、5、7、9 d,测定BMECs中αs1-酪蛋白、β-酪蛋白、κ-酪蛋白的基因表达量。结果表明,利用三维模式培养5 d的BMECsαs1-酪蛋白和κ-酪蛋白基因表达量极显著高于培养3、7、9 d(P<0.01);利用三维模式培养5 d的BMECsβ-酪蛋白的基因表达量极显著高于培养3、7 d(P<0.01),高于培养9 d,但差异不显著(P>0.05)。结果显示,三维模式下培养时间影响BMECs中αs1-酪蛋白、β-酪蛋白、κ-酪蛋白的基因表达量,本试验条件下最佳培养时间为5 d。
This study was conducted to investigate the effects of incubation time on casein gene expressions in bovine mammary epithelial cells （BMECs） in three-dimensional culture. Mammary parenchymal tissue was obtained from three healthy Holstein dairy cows aged from 3 to 5 years. The first generation of BMECs was purified and cultured in three-dimensional culture for 3, 5, 7 and 9 days, respectively. The expression levels of αs1-casein, β-casein and κ-casein genes in BMECs in three-dimensional culture were detected. The results showed that the expression levels of αs1-casein and κ-casein genes in BMECs in three-dimensional culture for 5 days were significantly higher than those for 3, 7 and 9 days （P〈0.01）. The expression level of β-casein gene in BMECs in three-dimensional culture for 5 days was higher than that for 3 （P〈0.01）, 7 （P〈0.01） and 9 days （P〉0.05）.The results indicate that the expression levels of αs1-casein, β-casein and κ-casein genes in BMECs are affected by incubation time in three-dimensional culture, and the optimal culture time is 5 days.
出处
《动物营养学报》
CAS
CSCD
北大核心
2013年第7期1526-1533,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家973项目"牛奶重要营养品质形成与调控机理研究"(2011CB100803)
关键词
奶牛乳腺上皮细胞
三维培养
酪蛋白
基因表达
培养时间
bovine mammary epithelial cells
three-dimensional culture
casein
gene expression
incubation time
