摘要
目的探讨慢性乙型肝炎患者外周血单个核细胞Toll样受体3(TLR3)的表达及其临床意义。方法分别采集慢性乙型肝炎患者和健康志愿者外周血,荧光定量PCR法检测血清HBV DNA复制水平;使用RT-PCR、流式细胞术以及免疫印迹技术分别检测外周血单个核细胞TLR3的mRNA、蛋白的表达;使用ELISA法检测血清中肿瘤坏死因子α(TNF-α)和干扰素β(IFN-β)水平。结果慢性乙型肝炎患者外周血单个核细胞中的TLR3表达显著低于健康志愿者,且降低水平与血清HBV DNA复制水平相关;慢性乙型肝炎患者外周血TNF-α、IFN-β浓度显著低于健康志愿者,且降低的水平与血清HBV DNA复制水平相关。结论慢性乙型肝炎患者外周血单个核细胞TLR3的表达与乙肝病毒的复制水平相关。
Objective To investigate the toll-like receptor 3 （TLR3） expressions in peripheral blood mononuclear cells of chronic hepatitis B patients and explore its clinical significance. Methods The peripheral blood were collect- ed from chronic hepatitis B patients and healthy volunteers, serum HBV DNA replication levels were detected by quantitative PCR; TLR3 mRNA and protein expression levels in peripheral blood mononuclear ceils were detected by RT-PCR, flow cytometry and Western blot respectively; the serum levels of TNF-α and IFN-β were measured by ELISA. Results The TLR3 expression in peripheral blood mononuclear cells of chronic hepatitis B patients was sig- nificantly lower than that in healthy volunteers and related to the level of serum HBV DNA replication; the serum TNF-α,IFN-β concentrations of chronic hepatitis B patients were significantly lower than that in healthy volunteers and related to the level of serum HBV DNA replication level. Condusions The TLR3 expression in peripheral blood mononuclear cells of chronic hepatitis B patients is associated with proliferation of hepatitis B virus.
出处
《基础医学与临床》
CSCD
北大核心
2013年第8期1028-1031,共4页
Basic and Clinical Medicine
