摘要
应用高压冷冻 冷冻替代技术研究欧洲赤松 (Pinussylvestris)树脂道细胞的超微结构并与传统方法进行比较 ,结果证明高压冷冻 冷冻替代技术不仅可提高树脂道细胞超微结构的保存 ,同时可减少树脂成分的损失 .具体表现为 ,细胞膜光滑平直 ,紧贴细胞壁 ,细胞器饱满 ,细胞质和细胞器基质更加浓厚均匀 ,高尔基体具有明显极性分化 ,潴泡间膜清晰 .树脂道细胞中树脂未形成浓密的黑色嗜锇滴 ,而是以浅灰色绒毛状结构存在 .上述特征表明经高压冷冻 冷冻替代制备的样品 ,其超微结构和组成成分的保存更接近于细胞的自然状态 .
Ultrastructure of resin duct cells of Pinus sylvestris was investigated with high pressure freezing(HPF) and freeze substitution(FS) and compared with the results of conventional chemical fixation methods(CF).With HPF FS method,plasma membranes were very smooth and appeared as straigth lines closely appressed to the cell wall.On the contrary,plasma membranes were often undulated and there was some space between the cell wall and the plasma membrane in CF samples,The organelles appeared turgid,matrices of cytoplasm and oragnelles were denser and more homogeneous distributed in HPF FS samples.The Golgi apparatus exhibited prominent differences in morphology and stainability from Cis to Trans face in HPF FS compared to CF,intercisternal elements were visualized in HPF FS specimens.Resin was often found to concentrate into small dark osmiophilic droplets with high electron density with CF,but with HPF FS,resin appeared as greyish osmiophilic material of lower electron density in floccule shape.These features implied that HPF FS samples retained more substances and improved preservation of ultrastructure closer to native state.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第5期82-86,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家教育部优秀年轻教师基金资助项目!(992 8)
关键词
超微结构
植物细胞
低温电镜技术
树脂道细胞
high pressure freezing
freezing substitution
resin duct
ultrastructure
