摘要
用60Co-γ射线辐射小麦品种河科2号的干种子,对M4代234个株系的蛋白质含量、湿面筋含量、沉降值、硬度的变异进行了分析。以超过群体均值±2×标准差为选择标准,筛选出6个蛋白质和湿面筋含量正向变异株系和3个负向变异株系。在M5代对它们的粉质参数进行了测定,并利用SDS-PAGE对其高分子量麦谷蛋白亚基(HMW-GS)进行了鉴定。结果表明,株系242的粉质图谱与粉质参数显著改变,株系240、108的HMW-GS组成也存在明显差异。亲本的HMW-GS组成为2+7+8+9+12,株系240、108的HMW-GS组成为2+7+8+12,说明在麦谷蛋白位点上发生了变异。
Dry seeds of wheat HEKE2 were radiated by ^60Co γ-ray with the dose of 300 Gy,and the genetic variation of protein content,gluten content,zeleny and hardness of 234 lines were analyzed in M4population.The contents of protein and gluten in 6 positive and 3 negative mutant lines were identified based on the selection criterion of over mean±2s.Further,their quality traits and farinogram parameters were detected,and high molecular weight glutenins（HMW-GS）were identified by SDS – PAGE in M5 generation.The result showed that the farinograph map and farinogram parameters of the mutant line 242 changed significantly,and HMW-GS composition of the parent was 2＋7＋8＋9＋12,while it was 2＋7＋8＋9 for the mutant line 108 and 240.The obvious change of HMW-GS composition suggests that mutations of glutenin locus may be occurred in the mutant lines.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2013年第4期575-579,共5页
Journal of Anhui Agricultural University
基金
安徽作物生物学省级实验室项目(10111406024)
安徽省自然科学基金(1208085MC57)
安徽省教育厅自然科学重点项目(KJ2012Z067)共同资助
