摘要
为探明陕西省果库中嗜酸耐热菌的分布情况,采用酸化的AAM培养基对陕西果库中的空气和苹果中的嗜酸耐热菌进行分离纯化,与参比菌株A.acidoterrestris DSM3922T进行细胞形态、菌落形态和生长条件、生理生化特征的比较分析,并以16S rDNA序列分析构建系统发育树,明确了8株分离菌的遗传学位置。分离株LC-4与标准菌的形态和生理生化特性有很大的相似性,16S rDNA序列分析表明LC-4与标准菌的同源性达99%,可判定为酸土脂环酸芽孢杆菌,其他7株属于芽孢杆菌。结果表明嗜酸耐热菌在果库中确有分布,可能对果汁质量造成威胁,需要加强控制。
In order to make sure the distribution of Alicyclobacillus from apple libraries in Shaanxi province, the Alicyclobacillus in apple and air were isolated and purified by using acidified AAM medium. The cell morphology, colony morphology, growing conditions, cultural characteristics and physiological characteristics were compared with the reference strain A. acidoterrestris DSM3922T. A phylogenetic tree based on 16S rDNA sequence analysis was constructed. The genetic location of eight isolated strains was clear. The isolated strain LC -4 was basically consistent with the reference stain Alicyclobacillus acidoterrestris DSM3922T in morphology and physiological characteristics. By 16S rDNA sequence analysis, isolated strain LC- 4 shared a homology of 99% with the reference strain, so it belonged to A. acidoterrestris and the other seven strains belonged to Bacillus ginsengihuimi. The results suggested AlicyclobaciUus was distributed in orchard soil, which might pose a threat to the quality of fruit juice, and needed to be controlled.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2013年第7期187-193,共7页
Transactions of the Chinese Society for Agricultural Machinery
基金
'十二五'国家科技支撑计划资助项目(2012BAD31B01)
国家自然科学基金面上项目(31071550
31171721)
农业部'948'项目
