摘要
目的探讨亚砷酸对卵蛋白诱导性关节炎滑膜细胞Caspase-3、细胞色素C的影响。方法将48只家兔随机分为正常对照组、模型对照组、亚砷酸低剂量组(1.0 mg.kg-1.d-1)、亚砷酸中剂量组(2.0 mg.kg-1.d-1)、亚砷酸高剂量组(4.0 mg.kg-1.d-1),激素组醋酸尼松龙(10 mg.d-1),每组8只。建立卵蛋白诱导关节炎兔模型,给药2周后,采用分光光度法检测滑膜细胞中的Caspase-3活性及免疫组化染色测定细胞色素C。结果检测结果显示模型组膝关节直径、表面温度均显著升高,与正常组比较由显著性差异(P<0.01);亚砷酸低剂量组、中剂量组、高剂量组均可显著性提高滑膜细胞Caspase-3、CytC水平(P<0.01或P<0.05)。结论推测亚砷酸作用于线粒体,使线粒体内的细胞色素C释放,激活Caspases,诱导滑膜细胞凋亡发生。
Objective To observe the effects of Sodium Arsenite （SDA） on the levels of Caspase-3 and CytC with the model of rabbit rheumatoid arthritis induced by ovalbumin. Methods Forty-eight rabbits were randomly divided into six groups ： normal control group, model group,low-dose of SDA group （ 1.0 mg · kg-1·d-1 ）, middle-dose of SDA group （2.0 mg · kg-1·d-1 ）, high-dose of SDA group（4.0 mg· kg-1·d-1 ） and prednisolone group（ 10 mg · d -1 ）. The model rabbit with rheumatoid arthritis induced by ovalbumin was established. After two weeks of therapy, the activity of Caspase-3 was assessed by Caspase-3 assay kit. CytC was examined by immunohistologica levaluation. Results After inducement, the articular diameters of both knees and the temperature of the articular skin showed an obvious increase,compared with normal group（P 〈0.01 ） ,Arsenious acid increased the key factors of the apoptotic pathway. There are differences compared with model group （ P 〈 0.01 ）. Conclusions Sodium arsenite has effect in mitochondria, resulting in Cyt- C release and activation of Caspases, and thus induce synoviocytes apoptosis.
出处
《安徽医药》
CAS
2013年第1期22-24,共3页
Anhui Medical and Pharmaceutical Journal
