摘要
目的:探讨肝细胞癌(HCC)组织中鼠双微体基因2(Mdm2)和磷酸化的丝氨酸/苏氨酸蛋白激酶(pAkt)的表达和临床意义。方法:采用免疫组织化学EliVision法测定70例HCC样本和40例癌旁组织样本中Mdm2和pAkt的表达,分析它们与临床病理特征之间的关系。结果:Mdm2在HCC中阳性率为34.3%,显著高于癌旁组织(P<0.01),其表达在肿瘤>5 cm和有淋巴结转移患者的HCC样本中显著增高(P<0.05)。pAkt在HCC中表达率为37.1%,显著高于癌旁组织(P<0.01),其表达在肿瘤大小、病理分级、TNM分期和淋巴结转移等临床病理参数上差异有统计学意义(P<0.05)。Mdm2与pAkt表达在HCC组织中呈正相关关系(r's=0.441,P<0.01)。结论:Mdm2和pAkt与HCC进展有关,有可能作为评价HCC预后的指标。
Objective：To explore the expression and clinical significance of murine double minute 2 （Mdm2） and phospho-serine/ threonine protein kinase（pAkt） in hcpatocellular carcinoma（HCC）. Methods：The protein levels of Mdm2 and pAkt were detected by EliVision immunohistochemistry in 70 HCC tissue samples and 40 tissues adjacent to carcinoma correlation between their expressions and clinicopathological features was analyzed. Results：The expression postive rate of Mdm2 in HCC tissue （34.3%） was significantly higher than that in tissues adjacent to carcinoma（ P 〈 0.01 ） ,its expression in tumor size more than 5 cm or lymphnode metastasis tissue significantly increased（ P 〈 0.05 ）. The expression postive rate of pAkt in HCC tissue （37.1%） was significantly higher than that in tissues adjacent to carcinoma（ P 〈 0.01 ） ,its expression differences in different tumor size ,pathologic grade ,TNM stage and lymphnode metastasis tissue were statistical significance （ P 〈 0.05 ）. The Mdm2 expression was positively related with pAkt expression in HCC tissue（ r＇, = 0.441 ,P 〈 0.01 ）. Conclusions：The expressions of Mdm2 and pAkt may be associated with the progression of HCC, which can serve as the index for evaluating the prognosis of HCC.
出处
《蚌埠医学院学报》
CAS
2013年第7期797-799,802,共4页
Journal of Bengbu Medical College
基金
安徽省自然科学基金资助项目(1208085MH170)
安徽省教育厅基金资助项目(KJ2013B147)
关键词
肝细胞癌
鼠双微体基因2
丝氨酸
苏氨酸蛋白激酶
hepatocellular carcinoma
nmrine double minute 2
serine/threonine protein kinase
