摘要
目的探讨乙醇诱导大鼠急性胃炎后一氧化氮合酶(nitric oxygen synthease,NOS)在胃黏膜的表达及意义。方法取30只成年雄性Wistar大鼠,随机分为单纯生理盐水灌胃组(A组)、单纯乙醇灌胃组(B组)、膈下迷走神经切断乙醇灌胃组(C组)、内脏大、小神经切断乙醇灌胃组(D组)和膈下迷走神经和内脏大、小神经均切断乙醇灌胃组(E组),每组6只。运用免疫组化技术显示NOS阳性神经元在各组胃黏膜中的表达情况。结果在C、E组动物的胃黏膜层及黏膜下层中只见稀疏的NOSⅠ阳性细胞,着色浅,而A、B、D组动物的胃黏膜层及黏膜下层中可见密集的NOSⅠ阳性细胞,着色较深;C、E组与A、B、D组的NOSⅠ阳性细胞数比较差异有统计学意义(P<0.05);而NOSⅡ阳性细胞在C、E组与B、D组的胃黏膜层及黏膜下层中的分布与NOSⅠ阳性细胞的分布相反。结论胃痛觉产生与过量NOS的生成密切相关。
Objective To study the expression and significance of nitric oxygen synthease （NOS） in gastric musa after ethanol-induced acute gastritis in rat. Methods 30 male Wistar mice were randomly divided in-to 5 groups, with 6 mice each： group A, gaster-poured by physiological solution; group B, gaster-poured by ethanol; group C, gaster-poured by ethanol after hypophrenic vagotomy; group D, gaster-poured by ethanol after big-small splanchnicotomy; group E, gaster-poured by ethanol after hypophrenic vagotomy and big-small splanchnicotomy. The distribution of NOS positive neuron in central nervous system was displayed and immunohistochemical staining method was used. Results The NOS I positive cells in mucosa of gastric body of groups C and E are sparse and light stained. The NOS Ⅰ positive cells in mucosa of gastric body of groups A, 13 and D are dense and deep-stained. The difference of the numbers of NOS Ⅰ positive cells between groups C, E and groups A, D, E has statistical significance （P 〈0.05）. The distribution of NOS Ⅱ positive cells in mucosa of gastric body in groups C, E and in groups B, D is contrary to that of NOS Ⅰ positive cells. Con- clusion Stomachache has a close relationship with the generation of excessive NOS.
出处
《新疆医科大学学报》
CAS
2013年第7期927-929,共3页
Journal of Xinjiang Medical University
基金
新疆医学动物模型研究重点实验室开放课题(XJDX1103-2011-03)
