摘要
为探索不同光源对结球甘蓝(Brassica oleracea var.Capitatal)不定芽再生的影响,采用LED红光、LED黄光、LED蓝光及对照荧光灯光源,选用12-1、12-2、12-3三个结球甘蓝材料进行了研究。结果表明:在LED红、黄、蓝色光源下,外植体愈伤组织的诱导率达100%;在不定芽的诱导中,LED红光的诱导率显著大于LED黄光,与荧光灯没有显著差异,平均诱导率高低依次为:红光>荧光>蓝光>黄光;在继代过程中,从再生系数看,LED红光与LED黄光存在显著差异,与荧光灯存在较明显差异,再生系数高低依次为:红光>蓝光>荧光灯>黄光;在根的诱导中,平均诱导率高低依次为:荧光>红光>蓝光>黄光,培养期间观测发现,LED光源下诱导的根粗壮,须根多,LED红光最明显;但在LED光源培养下有明显的褪绿和瓶壁凝露现象,LED红光下凝露最重;LED光源下先长愈伤组织再长腋芽,叶片变小且易碎,生长势弱,蓝光光源下节间伸长有明显的徒长现象;而荧光灯光源愈伤组织少,腋芽生长正常,没有出现以上现象。
In order to explore the effects of different light sources on the regeneration of cabbage adventitious buds, the three cabbage materials 12-1, 12-2 and 12-.3 were cultured respectively under the LED red light, LED yellow light, LED blue light and fluorescent light as control. The results show that the callus induction ratio was 100% under LED red, yellow and blue light, the induction rate of LED red light was significantly higher than that of LED yellow light, and there was no significant difference between LED red light and fluorescent light. The adventitious bud induction ratio ranged that under red light〉under fluorescent light〉under blue light〉under yellow light, the frequency of regeneration ranged that under red light〉under blue light〉under fluorescent light〉under yellow light, while the ratio of root induction ranged that under fluorescent light〉under red light〉under blue light〉under yellow light. The observation showed that under the LED light, the materials had more fibrous roots and the sidewall condesation phenomena, especially under the red light; the adventitious buds grew after the callus have been inducted, the leaves were small, creepy and had poor growth vigor, particularly, the materials showed stem elongation and spindling under the blue light. However, the callus induction ratio was low and the adventitious buds grew well under the fluorescent light.
出处
《天津农学院学报》
CAS
2013年第2期6-10,共5页
Journal of Tianjin Agricultural University
基金
国家科技计划项目"蔬菜抗病虫多基因分子标记辅助育种技术研究与优异种质创制"(2012AA100103)
江苏省农业科技自主创新资金项目"园艺种苗规模化生产温光技术集成创新及专用基质研发"(CX(11)2008)
江苏省农业三新工程项目"设施蔬菜水旱轮作防控连作障碍技术集成推广"(SXGC[2012]015)
关键词
LED光源
结球甘蓝
不定芽再生
LED light source
cabbage
adventitious bud regeneration
