摘要
目的:探讨隐匿性乙肝病毒表面抗原Y100C位点突变与乙肝病毒隐匿性感染的相关性。方法:分别构建含有隐匿性乙肝病毒表面抗原Y100C位点替换基因和野生型表面抗原基因的表达载体,转染HeLa细胞,培养一定时间后用ELISA方法检测细胞中HBsAg的表达情况,并进行统计学分析。结果:含有点突变基因的重组载体转染的细胞培养上清和提取物中HBsAg的表达量均低于含有野生型基因表达载体转染的细胞,差异具有统计学意义(P<0.05)。结论:HBsAg蛋白Y100C位点替换降低了HBsAg的合成及分泌,可能参与隐匿性异性肝炎的发生和发展,下一步的研究可结合HBV全基因的研究,以期更好的阐明隐匿性乙型肝炎的机制。
Objective： To discuss whether hepatitis B virus surface protein （HBsAg） variant Y100C substitution was associated with occult hepatitis B. Methods： We constructed two eukaryotic expression plasmids, containing a wild-type HBsAg gene and a HBsAg gene from a Y100C variant respectively, and compared their levels of HBsAg after transfection of HeLa cells by ELISA. Results： The extracellular and Cell extracts HBsAg levels detected with Y100C plasmid were lower than that with the wild-type plasmid（P〈0.05）. Conclusion： The Y100C substitution of HBsAg reduced HBsAg amounts, which may be involved in occurrence and development of occult hepatitis B. The next step studies can be combined with the complete genome of HBV, in order to better clarify the mechanism of occult hepatitis t3.
出处
《现代生物医学进展》
CAS
2013年第19期3645-3647,共3页
Progress in Modern Biomedicine
关键词
隐匿性乙型肝炎
表面抗原
真核表达
Occult Hepatitis B Virus
Surface protein
Eukaryotic expression
