摘要
从自主开发的近3000对烟草SSR引物中随机选出30对SSR引物,对38份晾晒烟种质资源的遗传关系进行了分析。在38份供试材料中共检测出173个等位基因,每对SSR引物可检测到的等位基因数为2～11个,平均为5.77个;遗传相似系数(GS)的变化范围为0.140～0.928,平均为0.534。表明38份晾晒烟的遗传多样性丰富,遗传差异较大,亲缘关系较远。聚类分析表明,在L1(GS=0.165)处可将38个品种分为2个类群,即晾晒烟类群和美国从烟草起源地收集的烟草(TI,tobaccoinstruction)类群;晾晒烟类群又可进一步分为4个亚类,其聚类结果与所期望的结果基本一致。表明SSR是一种有效、稳定和可靠的分子标记,能较好地从分子水平上揭示晾晒烟种质资源的遗传背景和亲缘关系。
The first phylogenesis of 38 sun/air-cured tobacco germplasms analyzed by simple sequence repeat（SSR） markers were reported in this study.30 primer pairs were randomly selected from a nearly total of 3000 selfdeveloped tobacco SSR primers.A total of 173 polymorphic alleles were amplified in 38 varieties,and the number of alleles detected by each SSR primer ranged from 2 to 11,with an average of 5.77 alleles per markers.Genetic similarity coefficient（GS） among the 38 varieties ranged from 0.140 to 0.928,with an average of 0.534,which indicated the genetic variation of the 38 varieties was quite large and the genetic diversity was much abundant in sun/aircured tobacco varieties.The result of cluster analysis based on SSR markers showed that 38 varieties could be clustered into 2 groups including sun/air-cured and TI tobacco groups at a relative low level of GS = 0.165.And the sun/air-cured group was further classified into 4 subgroups,which was basically according with expected case.The results showed that SSR was a stable,reliable,and valuable molecular marker system which was more efficient in generating accurate information on genetic background and relationship of tobacco,especially in sun/air-cured germplasms.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2013年第4期653-658,678,共7页
Journal of Plant Genetic Resources
基金
中国烟草总公司云南省公司项目(2010YN02
2011YN04)
关键词
晾晒烟
SSR标记
遗传多样性
亲缘关系
sun/air-cured tobaccos
simple sequence repeat（SSR） markers
genetic diversity
genetic relationship
