摘要
目的建立反相高效液相色潜法测定泽兰中木犀草素-7-0-β-D-葡萄糖苷的含量。方法采用AlltimaTM—C18（250minx4．6mm，51．Lm）；以乙腈-0．2％磷酸水溶=20：80进行等度洗脱，流速为1．0ml／min，检测波长为350mm，柱温为35℃。结果木犀草素-7-0-β-D-葡萄糖苷的线性范围为0．412-10．30μg（r=0．9999），平均回收率为100．25％。结论该方法结果准确、简便可行、重复性好，可为泽兰的质量控制提供依据。
Objective To establish a method for determination of luteolin-7-0-β-D-glucoside by HPLC. Methods Analysis was carried out on a AUtimaTM-C18 （250 mm x 4.6 iron, 5μm） eluted with water solution containing 0.2% phosphoric min-1. The UV detection wavelength was 350 nm, the temperature of column was 35 ℃. Results The linear response range was within 0.0412-1.030μg （r=-0.9999, n=5）. The recovery of it was 100.25%. Conclusion This method is simple, rapid and accurante, particularly suitable for the quality control of Lycopi Herba.
出处
《人参研究》
2013年第2期17-19,共3页
Ginseng Research
基金
江苏省重大科技项目(项目编号:BM2010610)
