摘要
目的探讨小鼠晕动病发病过程中内耳水通道蛋白的变化。方法多次旋转刺激后检测小鼠内耳中一系列水通道蛋白的mRNA水平;通过相关性分析观察内耳中水通道蛋白1的mRNA水平与个体晕反应指数之间的关系。通过内耳注射包含有水通道蛋白1的RNA干扰质粒的病毒,抑制内耳中水通道蛋白1的表达,观察其对小鼠晕反应指数的影响。结果多次旋转刺激后,小鼠的晕反应指数逐渐下降;小鼠内耳中水通道蛋白1的mRNA水平明显增加;通过RNA干扰的方法局部抑制小鼠内耳中水通道蛋白1的表达,发现会增加小鼠的晕反应指数。结论多次旋转刺激后,小鼠的晕反应指数的下降可能是缘于内耳中水通道蛋白1的表达增加。小鼠内耳中水通道蛋白1在晕动病的发生过程中起到重要作用。
OBJECTIVE To search the change of AQP1 in motion sickness （MS） in inner ear of mice. METHODS Rotation was used to simulate MS in mice. After repetitive stimulus of rotation, several aquaporins （AQPs） mRNA levels were analyzed using RT-PCR method. Relationship between AQP1 mRNA levels and MS index was analyzed. By using an RNAi approach to reduce the AQP1 protein expression in vivo,relationship between AQP1 mRNA level in inner ear of mice and MS index was studied. RESULTS After repetitive stimulus of rotation, the MS symptom was steadily alleviated in mice. AQP1 mRNA level was showed upregualted obviously, which was reconfirmed by Western blotting analysis. It was first observed that AQP1 knockdown in inner ear resulted in a significant increase of MS sensitivity in mice. CONCLUSION After repetitive stimulus of rotation, the alleviation of MS symptom in mice was, at least in part, due to the augmentation of AQP1 expression in inner ear. AQP1 in inner ear might be a novel target for the prevention or management of MS.
出处
《中国耳鼻咽喉头颈外科》
CSCD
2013年第6期302-305,共4页
Chinese Archives of Otolaryngology-Head and Neck Surgery
关键词
晕动病
小鼠
水孔蛋白质类
动物实验
逆转录聚合酶链反应
迷路
Motion Sickness
Mice
Aquaporins
Animal Experimentation
Reverse Transcriptase Polymerase Chain Reaction
Labyrinth
