摘要
将构建的单载体双基因Ad-BMP-2-IRES-HIF-1αmu转染到诱导的兔骨髓源性内皮祖细胞(endothelial progenitor cells,EPCs)后移植到股骨头缺血坏死(avascularnecrosis of the femoral head,ANFH)局部,检测其促进坏死区局部成血管和成骨能力。密度梯度离心法获取兔骨髓单个核细胞(mononuclearcells,MNCs),用M199培养基诱导MNCs为EPCs;通过细胞形态、特殊表面标记、摄取能力方面鉴定EPCs;将Ad-BMP-2-IRES-HIF-1αmu载体转染到EPCs,再将其移植入ANFH模型局部;分别于术后2周、4周处死模型,行坏死区成血管和成骨指标检测。结果显示:与对照组(B组)和空白组(C组)相比,实验组(A组)有较多新血管生成(P<0.05),B组与C组比较有统计学差异(P<0.05);组织学及BMP-2免疫组化检测,A组与B组和C组之间差异具有统计学意义(P<0.05),B组与C组之间差异无统计学意义(P>0.05)。综上所述,该实验提示移植转染Ad-BMP-2-IRES-HIF-1αmu的EPCs具有较强的成血管及成骨能力。
The induced EPCs were transfected by Ad-BMP-2-IRES-HIF-1αmu, and then transplanted into femoral head necrotic zone, the effect on osteogenesis and agiogenesis of necrosis zone was detected. MNCs were obtained by density gradient centrifugation method, and were induced into EPCs by M199 medium; EPCs were identified through cell morphology, specific surface markers and uptake ability. EPCs were transfected by Ad-BMP-2-IRES-HIF-1αmu, and then were transplanted into partial of ANFH. The model were enthanized at 2 and 4 weeks after operation, effect-ion on osteogenesis and agiogenesis of necrotic zone was detected. The results showed that there were more agiogenesis in group A than that in group B and C （P〈0.05）, the statistical difference was found between group B and C. The detection of histology and immunohistochemistry of BMP-2 showed that there was statistical difference between group A and B, group A and C （P〈0.05）. There was no statistical difference between group B and C （P〉0.05）. To sum up, there was an effection on osteogenesis and agiogenesis in EPCs after transfeced by Ad-BMP-2-IRES-HIF-1αmu.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2013年第7期967-972,共6页
Chinese Journal of Cell Biology
基金
辽宁省卫生厅"百千万人才工程"(批准号:2010921045)资助的课题~~
