摘要
目的探讨CK2抑制剂Emodin对人骨肉瘤细胞Saos-2增殖及侵袭力的影响。方法分别用CK2抑制剂Emodin、顺铂及联合两种药物处理Saos-2细胞,通过Western blot检测凋亡、CCK-8法检测细胞增殖;Transwell小室侵袭实验法检测细胞侵袭能力。结果 Emodin抑制CK2后可提高TAp73蛋白表达,激活其活性,促进Saos-2细胞凋亡;CCK-8法表明Emodin可以抑制Saos-2细胞增殖,且联合用药作用更明显;Transwell小室侵袭实验结果表明实验组均可抑制Saos-2细胞侵袭,穿膜细胞数分别为(57.23±3.52)、(52.06±4.54)和(32.83±3.79)个,且联合用药协同作用明显(P<0.05)。结论 CK2抑制剂Emodin能够抑制骨肉瘤Saos-2细胞的增殖和侵袭,为进一步探索采用小分子蛋白激酶抑制剂辅助治疗骨肉瘤细胞的新途径提供研究基础。
【Objective】 This study aimed at the effect of CK2 inhibitor on the invasion and proliferation of osteosarcoma Saos-2 cells.【Methods】 Western blot was used to detect detecting apoptosis.CCK-8 was used to detect the proliferation of osteosarcoma cells treated with Emodin and Cisplatin and combined treatment.Transwell invasion assay was used to measure the invasion of osteosarcoma cells.【Results】 Emodin inhibiting CK2 can be improved TAp73 protein expression,enhance the activity,promote Saos-2 cells apoptosis.CCK-8 showed that Emodin could inhibit the proliferation of osteosarcoma cells.And combined treatment＇s effect was more apparent.The experimental group all can inhibit Saos-2 cells to invade.Transwell invasion assays revealed that the number of cells was（57.23±3.52）,（52.06±4.54）,（32.83±3.79）.And the combination therapy of synergy was obviously（P 0.05）.【Conclusion】 CK2 inhibitor can inhibit Saos-2 cells invasion and proliferation.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第12期38-42,共5页
China Journal of Modern Medicine
基金
国家自然科学基金资助项目(No:81172555)
关键词
CK2抑制剂
人骨肉瘤细胞
肿瘤增殖
肿瘤侵袭
P73
CK2 inhibitor
human osteosarcoma cell
neoplasm invasiveness
neoplasm metastasis
p73
